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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
14
|
| pubmed:dateCreated |
1996-8-21
|
| pubmed:abstractText |
The use of porous, quaternized polyethylenimine-coated zirconia particles for the separation of nucleosides, nucleotides, oligonucleotides, and oligodeoxynucleotides is described. Separations of these biomolecules on quaternized PEI-zirconia are presented. Quaternized PEI-zirconia and a quaternary amine functionalized silica are compared chromatographically. Quaternized PEI-zirconia was able to separate oligonucleotides and oligodeoxynucleotides differing in length by a single nucleotide unit. It could also separate RNAs of the same length but differing by only one deoxynucleotide. In contrast, the silica-based phase was unstable at elevated temperatures (75 degrees C), and all retention toward anions was lost quite rapidly.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0003-2700
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
67
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2350-3
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1995
|
| pubmed:articleTitle |
High-performance anion-exchange chromatography of oligonucleotides and oligodeoxynucleotides on quaternized polyethylenimine-coated zirconia.
|
| pubmed:affiliation |
Department of Chemistry, University of Minnesota, Minneapolis 55455, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|