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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
1996-8-13
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pubmed:abstractText |
Rectal mucosal proliferation has been promoted as an intermediate marker for risk of colorectal neoplasia. Proliferating cell nuclear antigen (PCNA) immunohistochemistry has become a standard method to measure cell proliferation. Whole-crypt dissection may provide a technically simpler method for determining proliferation within an entire crypt. We conducted a study to assess the reliability (reproducibility) of whole-crypt dissection in 10 subjects. Reliability of whole-crypt dissection with the subject as the unit of observation was excellent. The intraclass correlation coefficient for subjects was 0.93. Biopsy-to-biopsy reliability was lower (r=0.86) and crypt-to-crypt reliability lower still (r = 0.35). There was poor correlation between measures of proliferation index using the two techniques (Kendall's tau = 0.13; P = 0.08). Compartment analysis based on the percentage of the total number of labeled cells appearing in each crypt quartile also did not demonstrate a significant correlation between the two measures. We conclude that PCNA labeling index and whole-crypt mitotic count are not comparable measures of rectal mucosal proliferation.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
1055-9965
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
4
|
pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
715-20
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8672987-Biopsy,
pubmed-meshheading:8672987-Cell Count,
pubmed-meshheading:8672987-Cell Division,
pubmed-meshheading:8672987-Colonoscopy,
pubmed-meshheading:8672987-Colorectal Neoplasms,
pubmed-meshheading:8672987-Female,
pubmed-meshheading:8672987-Humans,
pubmed-meshheading:8672987-Immunohistochemistry,
pubmed-meshheading:8672987-Intestinal Mucosa,
pubmed-meshheading:8672987-Male,
pubmed-meshheading:8672987-Proliferating Cell Nuclear Antigen,
pubmed-meshheading:8672987-Rectum,
pubmed-meshheading:8672987-Reproducibility of Results,
pubmed-meshheading:8672987-Risk Factors
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pubmed:articleTitle |
Comparison of rectal mucosal proliferation measured by proliferating cell nuclear antigen (PCNA) immunohistochemistry and whole crypt dissection.
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pubmed:affiliation |
Department of Medicine, University of North Carolina at Chapel Hill 27599-7080, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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