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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
1996-8-20
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pubmed:abstractText |
The human c-sis proto-oncogene promoter is transactivated by the human T-cell leukemia virus type 1 Tax protein in human Jurkat T-cells. Transactivation was >7-fold in Jurkat cells stably expressing the Tax protein (Jurkat-Tax) than in Jurkat E6.1 cells and was further enhanced in Jurkat-Tax cells stimulated with 12-O-tetradecanoylphorbol-13-acetate and the calcium ionophore, ionomycin. Deletion analysis showed that a 167-base pair promoter fragment retained full Tax responsiveness. Insertion of this minimal Tax-responsive region into a heterologous, minimal promoter resulted in approximately a 7-fold increase of transcriptional activation in the presence of Tax. Linker-scanning insertion analysis of this region identified Tax-responsive elements at nucleotides -64 to -45 (TRE1) and -34 to -15 (TATA box region). TRE1 contains a consensus binding site for the Sp family of transcription factors. The TATA box region corresponds to the TATA box and its 3'-neighboring sequence. Gel-shift and antibody supershift analysis of TRE1-binding proteins in unstimulated Jurkat E6.1 and Jurkat-Tax nuclear extracts identified Sp1 and Sp3 as the main TRE1 binding factors. Nuclear extracts from stimulated Jurkat E6.1 and Jurkat-Tax cells identified an additional TRE1 binding factor, Egr-1. These studies define a novel mechanism whereby Tax transactivates the c-sis promoter.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tax,
http://linkedlifedata.com/resource/pubmed/chemical/Ionomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-sis,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14584-90
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8662878-Base Sequence,
pubmed-meshheading:8662878-Binding Sites,
pubmed-meshheading:8662878-Cell Line,
pubmed-meshheading:8662878-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:8662878-Cloning, Molecular,
pubmed-meshheading:8662878-Consensus Sequence,
pubmed-meshheading:8662878-DNA-Binding Proteins,
pubmed-meshheading:8662878-Gene Products, tax,
pubmed-meshheading:8662878-Human T-lymphotropic virus 1,
pubmed-meshheading:8662878-Humans,
pubmed-meshheading:8662878-Ionomycin,
pubmed-meshheading:8662878-Kinetics,
pubmed-meshheading:8662878-Luciferases,
pubmed-meshheading:8662878-Molecular Sequence Data,
pubmed-meshheading:8662878-Mutagenesis,
pubmed-meshheading:8662878-Mutagenesis, Insertional,
pubmed-meshheading:8662878-Nuclear Proteins,
pubmed-meshheading:8662878-Oligodeoxyribonucleotides,
pubmed-meshheading:8662878-Platelet-Derived Growth Factor,
pubmed-meshheading:8662878-Promoter Regions, Genetic,
pubmed-meshheading:8662878-Proto-Oncogene Proteins,
pubmed-meshheading:8662878-Proto-Oncogene Proteins c-sis,
pubmed-meshheading:8662878-Proto-Oncogenes,
pubmed-meshheading:8662878-Recombinant Proteins,
pubmed-meshheading:8662878-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8662878-Sequence Deletion,
pubmed-meshheading:8662878-T-Lymphocytes,
pubmed-meshheading:8662878-TATA Box,
pubmed-meshheading:8662878-Tetradecanoylphorbol Acetate,
pubmed-meshheading:8662878-Transcriptional Activation,
pubmed-meshheading:8662878-Transfection,
pubmed-meshheading:8662878-Tumor Cells, Cultured
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pubmed:year |
1996
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pubmed:articleTitle |
c-sis/PDGF-B promoter transactivation by the Yax protein of human T-cell leukemia virus type 1.
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pubmed:affiliation |
Division of Molecular Oncology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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