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rdf:type |
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lifeskim:mentions |
umls-concept:C0017968,
umls-concept:C0019691,
umls-concept:C0019704,
umls-concept:C0021745,
umls-concept:C0024432,
umls-concept:C0026473,
umls-concept:C0036536,
umls-concept:C0036537,
umls-concept:C0086418,
umls-concept:C0123759,
umls-concept:C0205263,
umls-concept:C1514873
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pubmed:issue |
6
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pubmed:dateCreated |
1996-7-23
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pubmed:abstractText |
We studied the effects of the gp120 glycoprotein of human immunodeficiency virus type 1 on the expression of interleukin-12 (IL-12) in human monocytes and in monocyte-derived macrophages. Induction of the mRNA for both the p35 and p40 subunits of IL-12 was observed in both cell types after gp120 treatment. We then evaluated cytokine secretion by using an enzyme-linked immunosorbent assay which recognizes only the IL-12 heterodimer. No IL-12 was detected in monocytes/macrophages treated with gp120 alone. A consistent IL-12 secretion was found in macrophages primed with gamma interferon (IFN-gamma) and subsequently treated with gp120. Low levels of IL-12 were occasionally observed in IFN-gamma-primed monocytes stimulated with gp120. The greater response of macrophages than of monocytes to the priming effect of IFN-gamma was consistent with the finding that IFN-gamma induced a much stronger antiviral state to vesicular stomatitis virus in macrophages than in monocytes. These data indicate that gp120 is an inducer of IL-12 expression in monocytes/macrophages and that IFN-gamma is an essential cofactor for IL-12 secretion, especially in differentiated macrophages.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-1352483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-2223243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-2504877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-2550793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-3277274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-6422299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-7605994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-7722441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-7815507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-7903123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-7908232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-7908324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-7908633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-7911046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-8096699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-8103338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-8107259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-8392095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8648753-8464405
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4121-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8648753-Base Sequence,
pubmed-meshheading:8648753-Cells, Cultured,
pubmed-meshheading:8648753-HIV Envelope Protein gp120,
pubmed-meshheading:8648753-Humans,
pubmed-meshheading:8648753-Interferon-gamma,
pubmed-meshheading:8648753-Interleukin-12,
pubmed-meshheading:8648753-Macrophages,
pubmed-meshheading:8648753-Molecular Sequence Data,
pubmed-meshheading:8648753-Monocytes,
pubmed-meshheading:8648753-RNA, Messenger,
pubmed-meshheading:8648753-Recombinant Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Induction of interleukin-12 (IL-12) by recombinant glycoprotein gp120 of human immunodeficiency virus type 1 in human monocytes/macrophages: requirement of gamma interferon for IL-12 secretion.
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pubmed:affiliation |
Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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