Linked Life Data

8634426

Source:http://linkedlifedata.com/resource/pubmed/id/8634426

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1996-7-10
pubmed:abstractText
Single-cell suspensions of granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cells (G-PBMC) cultured in alpha minimal essential medium (alphaMEM) containing 10% fetal bovine serum formed multicellular aggregates within 24 hours. In six separate experiments, formation of aggregates appeared to be dependent on cell density per surface area, so that 5.8 +/- 1.3 aggregates formed per 1 x 10(5) cells when G-PBMC were cultured at densities greater than or equal to 1 x 10(5) cells/cm2. The frequency of aggregate formation was less than 1 per 10(5) cells when G-PBMC were cultured at densities less than 1 x 10(5) cells/cm2. Once formed, aggregates became adherent within 72 hours, and then, over the course of 21 days, released CD3/CD4/CD25-positive cells into the supernatant. This T-cell production peaked between days 7 and 14, reaching a total of 1,269 +/- 125.9 cells released per aggregate by day 21. Between days 14 and 21, the aggregates also generated macroscopic clusters of adherent mononuclear and giant multinucleated cells that stained positive for tartrate-resistant acid phosphatase (TRAP). At 4 weeks, the macroscopic foci coalesced into monolayers. Multinucleated TRAP-positive cells were distinguished from macrophage polykaryons by the absence of CD14 expression and the presence of osteoclast-specific membrane receptors for calcitonin and alphavbeta3-vitronectin. The osteoclast nature of these cells was further demonstrated by their ability to form resorption lacunae on dentine slices. Comparable osteoclast formation was not detected in cultures of normal marrow or normal nonmobilized peripheral blood.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1802-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8634426-Acid Phosphatase, pubmed-meshheading:8634426-Animals, pubmed-meshheading:8634426-Biological Markers, pubmed-meshheading:8634426-Bone Marrow, pubmed-meshheading:8634426-Bone Marrow Cells, pubmed-meshheading:8634426-Cattle, pubmed-meshheading:8634426-Cell Aggregation, pubmed-meshheading:8634426-Cell Differentiation, pubmed-meshheading:8634426-Cells, Cultured, pubmed-meshheading:8634426-Coloring Agents, pubmed-meshheading:8634426-Dentin, pubmed-meshheading:8634426-Granulocyte Colony-Stimulating Factor, pubmed-meshheading:8634426-Humans, pubmed-meshheading:8634426-Isoenzymes, pubmed-meshheading:8634426-Leukocytes, Mononuclear, pubmed-meshheading:8634426-Organ Specificity, pubmed-meshheading:8634426-Osteoclasts, pubmed-meshheading:8634426-Receptors, Calcitonin, pubmed-meshheading:8634426-Receptors, Vitronectin, pubmed-meshheading:8634426-Tooth Resorption
pubmed:year
1996
pubmed:articleTitle
Normal human peripheral blood mononuclear cells mobilized with granulocyte colony-stimulating factor have increased osteoclastogenic potential compared to nonmobilized blood.
pubmed:affiliation
Program in Transplantation Biology, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.