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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-5-3
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pubmed:abstractText |
Insect cells are routinely used for the production of receptor proteins. Expression of the Drosophila 5-HTdro1 serotonin receptor resulted in positive coupling of the receptor to adenylyl cyclase via the G(alpha)s G-protein subtype. The Drosophila 5-HTdro2B receptor stimulated the metabolism of inositol phospholipid via a pertussis toxin-insensitive G-protein, but exhibited no detectable inhibition of adenylyl cyclase. Immunoblot analysis of the endogenous G-proteins revealed that Sf9 cells lack the G-protein subtypes G(alpha i 1-3) and G(alpha)o, but express the subtype G(alpha)s and G(alpha)q.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
381
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
233-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8601462-Animals,
pubmed-meshheading:8601462-Baculoviridae,
pubmed-meshheading:8601462-Brain,
pubmed-meshheading:8601462-Cell Line,
pubmed-meshheading:8601462-Cyclic AMP,
pubmed-meshheading:8601462-Drosophila,
pubmed-meshheading:8601462-Drosophila Proteins,
pubmed-meshheading:8601462-Female,
pubmed-meshheading:8601462-GTP-Binding Proteins,
pubmed-meshheading:8601462-Humans,
pubmed-meshheading:8601462-Immunoblotting,
pubmed-meshheading:8601462-Inositol,
pubmed-meshheading:8601462-Kinetics,
pubmed-meshheading:8601462-Membrane Glycoproteins,
pubmed-meshheading:8601462-Myometrium,
pubmed-meshheading:8601462-Pregnancy,
pubmed-meshheading:8601462-Rats,
pubmed-meshheading:8601462-Receptors, Serotonin,
pubmed-meshheading:8601462-Recombinant Proteins,
pubmed-meshheading:8601462-Serotonin,
pubmed-meshheading:8601462-Spodoptera,
pubmed-meshheading:8601462-Transfection
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pubmed:year |
1996
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pubmed:articleTitle |
Functional characterisation of the Drosophila 5-HTdro1 and 5-HTdro2B serotonin receptors in insect cells: activation of a G(alpha) s-like protein by 5-HTdro1 but lack of coupling to inhibitory G-proteins by 5-HTdro2B.
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pubmed:affiliation |
School of Biological and Molecular Sciences, Oxford Brookes University, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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