Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1996-4-11
pubmed:databankReference
pubmed:abstractText
Molecular genetic analysis is used to characterize the AGT1 gene encoding an alpha-glucoside transporter. AGT1 is found in many Saccharomyces cerevisiae laboratory strains and maps to a naturally occurring, partially functional allele of the MAL1 locus. Agt1p is a highly hydrophobic, postulated integral membrane protein. It is 57% identical to Mal61p, the maltose permease encoded at MAL6, and is also a member of the 12 transmembrane domain superfamily of sugar transporters. Like Mal61p, Agt1p is a high-affinity, maltose/proton symporter, but Mal61p is capable of transporting only maltose and turanose, while Agt1p transports these two alpha-glucosides as well as several others including isomaltose, alpha-methylglucoside, maltotriose, palatinose, trehalose and melezitose. AGT1 expression is maltose inducible and induction is mediated by the Mal-activator. The sequence of the upstream region of AGT1 is identical to that of the maltose-inducible MAL61 gene over a 469 bp region containing the UASMAL but the 315 bp sequence immediately upstream of AGT1 shows no significant homology to the sequence immediately upstream of MAL61. The evolutionary origin of the MAL1 allele to which AGT1 maps and the relationship of AGT1 to other alpha-glucoside fermentation genes is discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1093-107
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8594329-Alleles, pubmed-meshheading:8594329-Amino Acid Sequence, pubmed-meshheading:8594329-Base Sequence, pubmed-meshheading:8594329-Carrier Proteins, pubmed-meshheading:8594329-Chromosome Mapping, pubmed-meshheading:8594329-Chromosomes, Fungal, pubmed-meshheading:8594329-Evolution, Molecular, pubmed-meshheading:8594329-Fermentation, pubmed-meshheading:8594329-Fungal Proteins, pubmed-meshheading:8594329-Gene Expression Regulation, Fungal, pubmed-meshheading:8594329-Genes, Fungal, pubmed-meshheading:8594329-Glucosides, pubmed-meshheading:8594329-Membrane Transport Proteins, pubmed-meshheading:8594329-Molecular Sequence Data, pubmed-meshheading:8594329-Monosaccharide Transport Proteins, pubmed-meshheading:8594329-Multigene Family, pubmed-meshheading:8594329-Saccharomyces cerevisiae, pubmed-meshheading:8594329-Saccharomyces cerevisiae Proteins, pubmed-meshheading:8594329-Sequence Alignment, pubmed-meshheading:8594329-Sequence Homology, pubmed-meshheading:8594329-Substrate Specificity, pubmed-meshheading:8594329-Symporters
pubmed:year
1995
pubmed:articleTitle
Characterization of AGT1 encoding a general alpha-glucoside transporter from Saccharomyces.
pubmed:affiliation
Queens College, Flushing, New York, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't