Linked Life Data

8590928

Source:http://linkedlifedata.com/resource/pubmed/id/8590928

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-4-4
pubmed:abstractText
Thiosulfate is a naturally occurring product of sulfur metabolism. Assays of urinary thiosulfate have been based on the reaction with cyanide to form thiocyanate. However, matrix interferences and background variation in endogenous thiocyanate excretion place serious constraints on this method for determination of physiological amounts of thiosulfate in urine. We describe a column-switching ion chromatographic separation for urinary thiosulfate that allows for sensitive and accurate detection by ion conductimetry. In 20 adult volunteers, we found a lower urinary thiosulfate (8.50 +/- 7.39 mumol/24 h, mean +/- S.D.) than others have described, although the upward skew of the results (median, 6.90; range, 0.84-32 mumol/24 h) was similar. However, we have not observed any of the interferences and the sensitivity of our technique (< 0.2 mumol/24 h) allows for detection of thiosulfate in all control samples. This sort of methodological improvement will be essential for any study of physiological thiosulfate metabolism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1572-6495
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
672
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-54
pubmed:dateRevised
2007-10-16
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Urinary thiosulfate determined by suppressed ion chromatography with conductimetric detection.
pubmed:affiliation
Department of Clinical Biochemistry, Banting Institute, University of Toronto, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't