| pubmed-article:8570651 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8570651 | lifeskim:mentions | umls-concept:C0028754 | lld:lifeskim |
| pubmed-article:8570651 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:8570651 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:8570651 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:8570651 | lifeskim:mentions | umls-concept:C0378310 | lld:lifeskim |
| pubmed-article:8570651 | lifeskim:mentions | umls-concept:C0162493 | lld:lifeskim |
| pubmed-article:8570651 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8570651 | pubmed:dateCreated | 1996-3-1 | lld:pubmed |
| pubmed-article:8570651 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8570651 | pubmed:abstractText | Like other adipocyte genes that are transcriptionally activated by CCAAT/enhancer binding protein alpha (C/EBP alpha) during preadipocyte differentiation, expression of the mouse obese (ob) gene is immediately preceded by the expression of C/EBP alpha. While the 5' flanking region of the mouse ob gene contains several consensus C/EBP binding sites, only one of these sites appears to be functional. DNase I cleavage inhibition patterns (footprinting) of the ob gene promoter revealed that recombinant C/EBP alpha, as well as a nuclear factor present in fully differentiated 3T3-L1 adipocytes, but present at a much lower level in preadipocytes, protects the same region between nucleotides -58 and -42 relative to the transcriptional start site. Electrophoretic mobility-shift analysis using nuclear extracts from adipose tissue or 3T3-L1 adipocytes and an oligonucleotide probe corresponding to a consensus C/EBP binding site at nucleotides -55 to -47 generated a specific protein-oligonucleotide complex that was supershifted by antibody against C/EBP alpha. Probes corresponding to two upstream consensus C/EBP binding sites failed to generate protein-oligonucleotide complexes. Cotransfection of a C/EBP alpha expression vector into 3T3-L1 cells with a series of 5' truncated ob gene promoter constructs activated reporter gene expression with all constructs containing the proximal C/EBP binding site (nucleotides -55 to -47). Mutation of this site blocked transactivation by C/EBP alpha. Taken together, these findings implicate C/EBP alpha as a transcriptional activator of the ob gene promoter and identify the functional C/EBP binding site in the promoter. | lld:pubmed |
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| pubmed-article:8570651 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8570651 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8570651 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8570651 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8570651 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8570651 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8570651 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:8570651 | pubmed:author | pubmed-author:LaneM DMD | lld:pubmed |
| pubmed-article:8570651 | pubmed:author | pubmed-author:MacDougaldO... | lld:pubmed |
| pubmed-article:8570651 | pubmed:author | pubmed-author:MandrupSS | lld:pubmed |
| pubmed-article:8570651 | pubmed:author | pubmed-author:HwangC SCS | lld:pubmed |
| pubmed-article:8570651 | pubmed:author | pubmed-author:GeimanD EDE | lld:pubmed |
| pubmed-article:8570651 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8570651 | pubmed:day | 23 | lld:pubmed |
| pubmed-article:8570651 | pubmed:volume | 93 | lld:pubmed |
| pubmed-article:8570651 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8570651 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8570651 | pubmed:pagination | 873-7 | lld:pubmed |
| pubmed-article:8570651 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8570651 | pubmed:meshHeading | pubmed-meshheading:8570651-... | lld:pubmed |
| pubmed-article:8570651 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8570651 | pubmed:articleTitle | Transcriptional activation of the mouse obese (ob) gene by CCAAT/enhancer binding protein alpha. | lld:pubmed |
