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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-3-1
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pubmed:databankReference |
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pubmed:abstractText |
Like other adipocyte genes that are transcriptionally activated by CCAAT/enhancer binding protein alpha (C/EBP alpha) during preadipocyte differentiation, expression of the mouse obese (ob) gene is immediately preceded by the expression of C/EBP alpha. While the 5' flanking region of the mouse ob gene contains several consensus C/EBP binding sites, only one of these sites appears to be functional. DNase I cleavage inhibition patterns (footprinting) of the ob gene promoter revealed that recombinant C/EBP alpha, as well as a nuclear factor present in fully differentiated 3T3-L1 adipocytes, but present at a much lower level in preadipocytes, protects the same region between nucleotides -58 and -42 relative to the transcriptional start site. Electrophoretic mobility-shift analysis using nuclear extracts from adipose tissue or 3T3-L1 adipocytes and an oligonucleotide probe corresponding to a consensus C/EBP binding site at nucleotides -55 to -47 generated a specific protein-oligonucleotide complex that was supershifted by antibody against C/EBP alpha. Probes corresponding to two upstream consensus C/EBP binding sites failed to generate protein-oligonucleotide complexes. Cotransfection of a C/EBP alpha expression vector into 3T3-L1 cells with a series of 5' truncated ob gene promoter constructs activated reporter gene expression with all constructs containing the proximal C/EBP binding site (nucleotides -55 to -47). Mutation of this site blocked transactivation by C/EBP alpha. Taken together, these findings implicate C/EBP alpha as a transcriptional activator of the ob gene promoter and identify the functional C/EBP binding site in the promoter.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-1339452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-1373117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-14824537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-1568242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-1738600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-1993067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-2006196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-2059845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-2404278,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-2606350,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-271968,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-4705382,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-7372608,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-7984236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-8001151,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8570651-8346250
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
873-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8570651-Animals,
pubmed-meshheading:8570651-Base Sequence,
pubmed-meshheading:8570651-Binding Sites,
pubmed-meshheading:8570651-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:8570651-DNA Footprinting,
pubmed-meshheading:8570651-DNA-Binding Proteins,
pubmed-meshheading:8570651-Enhancer Elements, Genetic,
pubmed-meshheading:8570651-Leptin,
pubmed-meshheading:8570651-Luciferases,
pubmed-meshheading:8570651-Mice,
pubmed-meshheading:8570651-Molecular Sequence Data,
pubmed-meshheading:8570651-Nuclear Proteins,
pubmed-meshheading:8570651-Promoter Regions, Genetic,
pubmed-meshheading:8570651-Protein Binding,
pubmed-meshheading:8570651-Protein Biosynthesis,
pubmed-meshheading:8570651-Proteins,
pubmed-meshheading:8570651-Recombinant Fusion Proteins,
pubmed-meshheading:8570651-Transcription, Genetic,
pubmed-meshheading:8570651-Transcriptional Activation
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pubmed:year |
1996
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pubmed:articleTitle |
Transcriptional activation of the mouse obese (ob) gene by CCAAT/enhancer binding protein alpha.
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