Linked Life Data

8568766

Source:http://linkedlifedata.com/resource/pubmed/id/8568766

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-3-6
pubmed:abstractText
Invasive adenylate cyclase (iAC) reversibly inhibits spontaneous maturation of cumulus-enclosed bovine oocytes by increasing the intracellular concentration of cAMP, [cAMP]i. In this study, physiological aspects of maintaining meiotic arrest in bovine oocytes by iAC were investigated. The maintenance of germinal vesicle arrest by iAC in both cumulus-enclosed and denuded bovine oocytes was concentration dependent (r2 = 0.857). Denuded bovine oocytes were more sensitive to maintenance of meiotic arrest by iAC then were cumulus-enclosed oocytes. At the highest concentration, 70% of the cumulus-enclosed and 90% of the denuded bovine oocytes were maintained in meiotic arrest. The iAC increased [cAMP]i in both intact cumulus-oocyte complexes and enclosed oocytes in a concentration-dependent manner (r2 = 0.795). Cumulus-enclosed oocytes maintained in meiotic arrest by iAC retained developmental competence when subsequently cultured in iAC-free medium and then fertilized. The [cAMP]i in bovine complexes decreased precipitously upon release from follicles and remained low for the next 125 min. However, the [cAMP]i of the enclosed oocytes did not change. Bovine oocytes commit to undergo meiosis in a progressive manner. Approximately 10% of the oocytes were already committed when aspirated. This proportion increased to 40% at 2 h and 70% at 5 h. Use of two inhibitors of cAMP-dependent protein kinase A provided further evidence that cAMP functions in mediating meiotic arrest in bovine oocytes. Bovine oocytes, therefore, are sensitive to different cAMP concentrations, and are developmentally competent after iAC-induced arrest, and complexes containing oocytes exhibit a decrease in [cAMP]i before spontaneous maturation. These results suggest that maintenance of meiotic arrest by iAC is accomplished through modulation of cellular machinery, and regulation of oocyte maturation by [cAMP]i may be physiologically relevant.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-4251
pubmed:author
pubmed:issnType
Print
pubmed:volume
105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-45
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:8568766-Adenylate Cyclase, pubmed-meshheading:8568766-Analysis of Variance, pubmed-meshheading:8568766-Animals, pubmed-meshheading:8568766-Bordetella pertussis, pubmed-meshheading:8568766-Cattle, pubmed-meshheading:8568766-Cells, Cultured, pubmed-meshheading:8568766-Culture Media, pubmed-meshheading:8568766-Cyclic AMP, pubmed-meshheading:8568766-Cyclic AMP-Dependent Protein Kinases, pubmed-meshheading:8568766-Dose-Response Relationship, Drug, pubmed-meshheading:8568766-Female, pubmed-meshheading:8568766-Intracellular Fluid, pubmed-meshheading:8568766-Isoquinolines, pubmed-meshheading:8568766-Oocytes, pubmed-meshheading:8568766-Oogenesis, pubmed-meshheading:8568766-Prophase, pubmed-meshheading:8568766-Sulfonamides, pubmed-meshheading:8568766-Thionucleotides, pubmed-meshheading:8568766-Time Factors
pubmed:year
1995
pubmed:articleTitle
Maintenance of meiotic arrest by increasing [cAMP]i may have physiological relevance in bovine oocytes.
pubmed:affiliation
Department of Meat and Animal Science, University of Wisconsin, Madison 53706, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't