Linked Life Data

8537106

Source:http://linkedlifedata.com/resource/pubmed/id/8537106

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1996-2-6
pubmed:abstractText
The antibody response to horse cytochrome c (cyt.c) in BALB/c mice developed slowly and a substantial production of IgG antibodies was observed only 26-30 days after immunization. Lymph node cells (LNC) of unimmunized mice proliferated weakly in response to both native cyt.c and its synthetic peptides. On day 8 after immunization, LNC could not be stimulated with native cyt.c and peptide 92-104. However, they did proliferate in response to cyt.c peptides 1-6, 1-13, 2-13, 14-22, 46-56, 57-77, 61-77 and 61-69 which are closely related in horse and mouse cyt.c. On day 26, both native cyt.c and the peptides, including 92-104, were equally active in stimulating LNC proliferation. Both plastic-adherent and cyt.c-specific cells panned from day 8 cells enhanced the response of unprimed cells to native cyt.c. Elimination of B cells demonstrated that primary recognition of cyt.c was mediated, at least partly, by non-specific antigen-presenting cells (APC) while later B cells of additional specificities were involved. It is concluded that immunization with horse cyt.c initiated an autoimmune response resulting in T-dependent anergy. Peptide determinants processed by non-specific APC stimulated corresponding autoreactive T cells. Specific B cells which appeared as a result of the response maturation processed successfully the immunodominant epitope and finally mediated proliferative and antibody responses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0165-2478
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
87-92
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
The immune response to cytochrome c in BALB/c mice is delayed due to inability of their non-specific antigen-presenting cells to provide its immunodominant epitope.
pubmed:affiliation
Palladin Institute of Biochemistry, Kiev, Ukraine.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't