Linked Life Data

8395537

Source:http://linkedlifedata.com/resource/pubmed/id/8395537

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-9-27
pubmed:abstractText
A polymerase chain reaction (PCR) technique was used to detect the presence of foot-and-mouth disease virus (FMDV) in oesophageal-pharyngeal(OP) samples from experimentally infected steers. Ten-fold dilutions of OP samples were also diluted, inoculated onto lamb kidney cell cultures, and incubated overnight. The cultures that did not show overt cytopathogenic effects (CPE) of FMDV infection were frozen and thawed; both the fluid and the cell pellet were tested by the PCR technique. The PCR detected FMDV in the fluids of 57% of the cell cultures inoculated with 2-20 tissue culture infective doses-50% (TCID50) and of 33% of cell cultures inoculated with the 0.4-2 TCID50. The PCR detected FMDV in cell pellets of all cell cultures inoculated with 20 TCID50, of 71% of cell cultures inoculated with 2-20 TCID50 and of 50% of cell cultures inoculated with 0.2-2 TCID50. A diagnostic scheme using PCR and cell culture that would provide rapid and sensitive detection of FMDV in OP samples is proposed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-6
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
The detection of foot-and-mouth disease virus in oesophageal-pharyngeal samples by a polymerase chain reaction technique.
pubmed:affiliation
USDA, VS, APHIS, NVSL, FADDL, Greenport, NY 11944.
pubmed:publicationType
Journal Article