Linked Life Data

8184426

Source:http://linkedlifedata.com/resource/pubmed/id/8184426

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-6-10
pubmed:abstractText
Recent morphological studies from our laboratory have shown that copper deficiency-induced pancreatic involution in rats is secondary to apoptosis (M. S. Rao, A. V. Yeldandi, V. Subbarao, and J. K. Reddy, 1993, Am. J. Pathol. 142, 1952-1957). To corroborate the morphological findings, we have examined pancreases from copper-deficient rats for expression of sulfated glycoprotein-2 (SGP-2) mRNA and DNA fragmentation, which are considered as specific markers of apoptosis. Agarose gel electrophoresis of the DNA extracted from pancreases of rats maintained on copper deficient diet (CUDD) for 5 and 7 weeks showed characteristic "ladder" pattern, whereas DNA from control rats and rats maintained on CUDD for 3 weeks showed no fragmentation. These findings correlated well with the histological changes. Northern blot analysis of total RNA revealed a marked increase in the expression of SGP-2 mRNA at 5 weeks followed by a gradual decrease at 6 and 7 weeks. These results further support that the mechanism of copper deficiency-induced pancreatic involution is through apoptosis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:volume
126
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
174-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Increased expression of sulfated glycoprotein-2 and DNA fragmentation in the pancreas of copper-deficient rats.
pubmed:affiliation
Department of Pathology, Northwestern University Medical School, Chicago, Illinois 60611.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't