Linked Life Data

8133784

Source:http://linkedlifedata.com/resource/pubmed/id/8133784

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1994-4-15
pubmed:abstractText
Genetic heterogeneity in response to genotoxic carcinogens requiring metabolic conversion has been studied in Drosophila, using seven different genotypes in combination with the w/w+ eye mosaic assay for mitotic recombination. The set of tester strains examined consisted of four wild-type laboratory strains (Berlin-K, BK; Oregon-K, OK; Leiden-S, LS; and 91-C), and three DDT-resistant strains (91-R; Hikone-R, HR; and Haag-79, HG). Drosophila larvae heterozygous for the wild-type report gene w+ were exposed to benz[a]anthracene (BA), benzo[a]pyrene (BP), 9,10-dimethyl-anthracene (DA), monocrotaline (MC), N-nitrosodimethylamine (DMN) or vinyl bromide (VBr). The primary conclusion regarding this study is the up to 60-fold variation between different genotypes in mosaic spot frequencies induced by those procarcinogens and the non-existence of just one genotype to function as a 'super-strain' in the activation of different classes of procarcinogens. By contrast, the seven Drosophila strains show a similar response to the direct-acting agent methyl methanesulphonate. Among the six procarcinogens selected for this study, DMN and MC are readily detectable in all the distinct genotypes. Less satisfactory in terms of test performance are the either negative or weak test responses with BA in all four-wild type strains (LS, BK, OK and 91-C), and the weakly positive results for vinyl bromide in LS, BK and OK. The best overall test response was provided by strains HG and HR. It is concluded that the combined application of strains HG and HR safely and reliably should detect somatic cell recombinagens in the Drosophila w/w+ system.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0267-8357
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
543-51
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Strong intra-species variability in the metabolic conversion of six procarcinogens to somatic cell recombinagens in Drosophila.
pubmed:affiliation
Laboratorio de Génetica, Facultad de Ciencias, UNAM, Coyoacan 04510, Mexico DF.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't