Linked Life Data

8102346

Source:http://linkedlifedata.com/resource/pubmed/id/8102346

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-9-14
pubmed:databankReference
pubmed:abstractText
A cDNA clone encoding the proliferating cell nuclear antigen (PCNA) has been isolated from a Brassica napus apical meristem cDNA library. The putative full-length cDNA contains an open reading frame of 1004 nucleotides, which predicts a protein of 263 amino acids (M(r) = 29,231). Sequence analysis has revealed that the plant PCNA exhibits 81.6% amino acid similarity with the human PCNA. Genomic Southern blot analysis indicates the presence of at least two copies of PCNA per genome. The B. napus PCNA mRNA (1.0 kb) was expressed in rapidly dividing tissues such as flower buds, apical meristems, and young leaves, while mature stem and fully expanded leaves showed significantly lower levels of PCNA transcript. The B. napus PCNA cDNA was expressed in Escherichia coli as a fusion protein with glutathione-S-transferase (GST) in the bacterial expression vector pGEX-2T. A broad specificity monoclonal antibody raised against rabbit PCNA cross-reacted with the GST-PCNA fusion peptide but not with the GST moiety alone. This antibody also recognized the human PCNA (36 kDa) polypeptide, confirming the structural similarities between the human and plant PCNA. The high degree of structural conservation of PCNA from such diverse organisms as humans and higher plants suggests that the plant PCNA may function in a manner analogous to that found in mammals with respect to plant cell DNA replication. Such conservation suggests that PCNA is also a critical component of the plant cell DNA replication complex.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0831-2796
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
459-66
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Molecular cloning and expression of a cDNA encoding the proliferating cell nuclear antigen from Brassica napus (oilseed rape).
pubmed:affiliation
Department of Biological Sciences, University of Calgary, Alta., Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't