Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1994-12-29
|
| pubmed:databankReference | |
| pubmed:abstractText |
The metallothionein (MT) I and II genes were isolated from Chinese hamster cells and sequenced. The MT-II gene is located about 6 kb upstream of the MT-I gene and their arrangement is similar to those of the mouse and rat MT genes. The sequence of the Chinese hamster MT-I gene is highly homologous to those of the mouse and rat, particularly in their promoter regions of MT-I. However, the promoter region of MT-II has less homology with those of the mouse and rat due t to insertions and deletions. The MT-I and MT-II genes were equally amplified 4-8-times in the Cd-resistant Chinese hamster cells, suggesting that both genes are included in the same amplification unit. Cytogenetic analysis of Cd-resistant cells by in situ hybridization showed that they are randomly integrated into multiple sites on the chromosomes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0006-3002
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
22
|
| pubmed:volume |
1219
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
581-91
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:7948015-Amino Acid Sequence,
pubmed-meshheading:7948015-Animals,
pubmed-meshheading:7948015-Base Sequence,
pubmed-meshheading:7948015-Cadmium,
pubmed-meshheading:7948015-Cells, Cultured,
pubmed-meshheading:7948015-Chromosome Mapping,
pubmed-meshheading:7948015-Cloning, Molecular,
pubmed-meshheading:7948015-Cricetinae,
pubmed-meshheading:7948015-Cricetulus,
pubmed-meshheading:7948015-DNA, Complementary,
pubmed-meshheading:7948015-Drug Resistance,
pubmed-meshheading:7948015-Gene Amplification,
pubmed-meshheading:7948015-Genome,
pubmed-meshheading:7948015-In Situ Hybridization,
pubmed-meshheading:7948015-Metallothionein,
pubmed-meshheading:7948015-Mice,
pubmed-meshheading:7948015-Molecular Sequence Data,
pubmed-meshheading:7948015-Muridae,
pubmed-meshheading:7948015-Promoter Regions, Genetic,
pubmed-meshheading:7948015-Rats,
pubmed-meshheading:7948015-Sequence Homology, Nucleic Acid
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Sequence homology of Chinese hamster metallothionein genes I and II to those of the mouse and rat, and their amplification in Cd-resistant cells.
|
| pubmed:affiliation |
Division of Geriatric Health Science, National Institute of Health and Nutrition, Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|