Linked Life Data

781255

Source:http://linkedlifedata.com/resource/pubmed/id/781255

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1976-9-25
pubmed:abstractText
Two methods are described for fracturing unfixed, uncryoprotected mouse liver that had been frozen by contact with a copper block at liquid nitrogen temperature. The best method involved precise fracturing of a few microns of surface tissue with a cooled glass knife, using the Cryokit attachment of the LKB III ultramicrotome. The resulting replicas were free from electron microscopically-visible ice crystals. A noticeable feature of replicas from unfixed, uncryoprotected tissue was the extent of plastic deformation of certain cellular structures. Cytoplasmic macromolecules, and to a lesser extent intramembranous particles, often appeared to be 'stretched' to form fibrils.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-2720
pubmed:author
pubmed:issnType
Print
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
261-71
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
A copper block method for freezing non-cryoprotected tissue to produce ice-crystal-free regions for electron microscopy. II. Evaluation using freeze fracturing with a cryo-ultramicrotome.
pubmed:publicationType
Journal Article