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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
1995-5-15
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pubmed:abstractText |
Several guanine nucleotide-binding protein-coupled receptors are known to be rapidly phosphorylated after agonist exposure. In this study we show that the gastrin-releasing peptide receptor (GRP-R) is rapidly phosphorylated in response to agonist exposure. When [32P]orthophosphate-labeled cells were exposed to bombesin, the receptor was maximally phosphorylated on serine and threonine residues within 1 min. Although addition of 12-O-tetradecanoylphorbol 13-acetate also resulted in phosphorylation of the GRP-R, elimination of protein kinase C activity using the inhibitor 7-hydroxystaurosporine did not prevent bombesin-induced GRP-R phosphorylation. We conclude that a kinase other than protein kinase C is principally responsible for the rapid, agonist-induced phosphorylation of the GRP-R.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8217-24
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7713928-Amino Acid Sequence,
pubmed-meshheading:7713928-Animals,
pubmed-meshheading:7713928-Bombesin,
pubmed-meshheading:7713928-Cells, Cultured,
pubmed-meshheading:7713928-Enzyme Activation,
pubmed-meshheading:7713928-Immune Sera,
pubmed-meshheading:7713928-Mice,
pubmed-meshheading:7713928-Mice, Inbred BALB C,
pubmed-meshheading:7713928-Molecular Sequence Data,
pubmed-meshheading:7713928-Phosphorylation,
pubmed-meshheading:7713928-Protein Kinase C,
pubmed-meshheading:7713928-Protein Kinases,
pubmed-meshheading:7713928-Receptors, Bombesin,
pubmed-meshheading:7713928-Tetradecanoylphorbol Acetate,
pubmed-meshheading:7713928-Transfection
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pubmed:year |
1995
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pubmed:articleTitle |
The gastrin-releasing peptide receptor is rapidly phosphorylated by a kinase other than protein kinase C after exposure to agonist.
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pubmed:affiliation |
Laboratory of Biological Chemistry, NCI National Institutes of Health, Bethesda, Maryland 20892-4255, USA.
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pubmed:publicationType |
Journal Article
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