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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-4-24
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pubmed:abstractText |
Endothelin (ET)-2 is a 21 residue vasoactive peptide which is biosynthesized from big ET-2(1-38) by a specific cleavage at Trp21-Val22 with an ET converting enzyme (ECE). To identify an ECE in ACHN (human renal adenocarcinoma) cells which produce ET-2, we have cloned and sequenced a novel cDNA encoding a human ECE in ACHN (hAECE). It encodes a 770 amino acid protein with a zinc-binding motif and a single membrane spanning region. The sequences of nucleic acids and amino acids from Leu45 to Trp770 of hAECE are identical to those from Leu33 to Trp758 of a human ECE in HUVEC (hHECE). The sequences in the amino-terminal moiety are divergent between hAECE and hHECE. Based on the difference of the amino-terminal amino acid sequences, ECEs reported so far, can be classified into two isoforms. These results strongly suggest that an alternative splicing might occur in the 5'-terminal region of the ECE pre-mRNA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-291X
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
208
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
721-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7695628-Adenocarcinoma,
pubmed-meshheading:7695628-Alternative Splicing,
pubmed-meshheading:7695628-Amino Acid Sequence,
pubmed-meshheading:7695628-Animals,
pubmed-meshheading:7695628-Aspartic Acid Endopeptidases,
pubmed-meshheading:7695628-Base Sequence,
pubmed-meshheading:7695628-Cattle,
pubmed-meshheading:7695628-Cloning, Molecular,
pubmed-meshheading:7695628-DNA, Complementary,
pubmed-meshheading:7695628-Endothelins,
pubmed-meshheading:7695628-Humans,
pubmed-meshheading:7695628-Kidney Neoplasms,
pubmed-meshheading:7695628-Metalloendopeptidases,
pubmed-meshheading:7695628-Molecular Sequence Data,
pubmed-meshheading:7695628-Rats,
pubmed-meshheading:7695628-Sequence Alignment,
pubmed-meshheading:7695628-Sequence Homology, Amino Acid,
pubmed-meshheading:7695628-Tissue Distribution
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pubmed:year |
1995
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pubmed:articleTitle |
Cloning and sequencing of a human endothelin converting enzyme in renal adenocarcinoma (ACHN) cells producing endothelin-2.
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pubmed:affiliation |
Division of Cardiovascular Biology, Chiba University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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