Linked Life Data

7591302

Source:http://linkedlifedata.com/resource/pubmed/id/7591302

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-12-6
pubmed:abstractText
Peptide specificity of cultured tumor-infiltrating lymphocytes (TIL) was systematically investigated in a group of HLA-A2.1+ metastatic melanoma patients consecutively referred to our department for surgical treatment. Seven samples from 6 patients were studied. All surgical specimens showed evidence of gp 100, MART-1/Melan-A and Tyrosinase gene expression as detectable by reverse PCR (rPCR). Cultured TIL from 2 patients displayed cytotoxic activity against autologous or HLA-matched EBV-transformed cells previously pulsed with MART-1/Melan-A27-35 peptide. In contrast, no CTL activity against gp100(280-288) or tyrosinase1-9 peptides could be observed. TIL were then repeatedly stimulated in vitro with the same peptides. After 6 restimulation courses at weekly intervals, specific recognition of gp100(280-288) and MART-1/Melan-A peptides was detectable in 3 and 5 TIL populations, respectively. In one case Tyrosinase1-9-specific CTL could be demonstrated. Two TIL populations from metastases resected from a melanoma patient at 6 months' distance showed a different peptide specificity pattern, and no specific CTL could be generated from simultaneously sampled peripheral blood mononuclear cells (PBMC). All peptide-specific CTL populations also displayed significant cytotoxic activity against HLA-A2.1 matched melanoma cell lines expressing the antigens under investigation. Our data indicate that CTL specific for MART-Melan-A27-35, gp100(280-288) or Tyrosinase1-9 peptides could be expanded with varying frequency from TIL derived from 4 out of 6 HLA-A2.1+ patients whose tumors expressed the genes encoding these tumor-associated antigens (TAA).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0020-7136
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-15
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:7591302-Adult, pubmed-meshheading:7591302-Aged, pubmed-meshheading:7591302-Aged, 80 and over, pubmed-meshheading:7591302-Alleles, pubmed-meshheading:7591302-Amino Acid Sequence, pubmed-meshheading:7591302-Antigens, Neoplasm, pubmed-meshheading:7591302-Base Sequence, pubmed-meshheading:7591302-Cell Transformation, Viral, pubmed-meshheading:7591302-Cytotoxicity, Immunologic, pubmed-meshheading:7591302-Epitopes, pubmed-meshheading:7591302-Female, pubmed-meshheading:7591302-Granulocytes, pubmed-meshheading:7591302-HLA-A2 Antigen, pubmed-meshheading:7591302-Herpesvirus 4, Human, pubmed-meshheading:7591302-Humans, pubmed-meshheading:7591302-Isoantigens, pubmed-meshheading:7591302-Lymphocyte Activation, pubmed-meshheading:7591302-Lymphocytes, Tumor-Infiltrating, pubmed-meshheading:7591302-Male, pubmed-meshheading:7591302-Melanoma, pubmed-meshheading:7591302-Membrane Glycoproteins, pubmed-meshheading:7591302-Middle Aged, pubmed-meshheading:7591302-Molecular Sequence Data, pubmed-meshheading:7591302-Monophenol Monooxygenase, pubmed-meshheading:7591302-Neoplasm Proteins, pubmed-meshheading:7591302-Peptides, pubmed-meshheading:7591302-Phenotype, pubmed-meshheading:7591302-Polymerase Chain Reaction, pubmed-meshheading:7591302-T-Lymphocytes, Cytotoxic, pubmed-meshheading:7591302-gp100 Melanoma Antigen
pubmed:year
1995
pubmed:articleTitle
Peptide-specific CTL in tumor infiltrating lymphocytes from metastatic melanomas expressing MART-1/Melan-A, gp100 and Tyrosinase genes: a study in an unselected group of HLA-A2.1-positive patients.
pubmed:affiliation
Department of Surgery, University of Basel, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't