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rdf:type |
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lifeskim:mentions |
umls-concept:C0007585,
umls-concept:C0030685,
umls-concept:C0040690,
umls-concept:C0086597,
umls-concept:C0206116,
umls-concept:C0391871,
umls-concept:C0591833,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1456820,
umls-concept:C1963578
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pubmed:issue |
3
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pubmed:dateCreated |
1995-12-28
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pubmed:abstractText |
Tumor necrosis factor (TNF)-alpha and transforming growth factor (TGF)-beta produced by glial cells have been proposed bo play a role in various neurodenegerative diseases. The interaction of these two cytokines, however, is unknown. We tested the hypothesis that the TNF-alpha released from lipopolysaccharide (LPS)-treated murine microglial cells would stimulate the release of TGF-beta, which in turn would control TNF-alpha production. Treatment of murine microglial cell cultures with LPS resulted in an acute release of TNF-alpha (peak by 8 hr) followed by delayed release of bioactive TGF-beta (peak by 48 hr). Anti-TNF-alpha antibody significantly inhibited LPS-stimulated TGF-beta production, suggesting the involvement of TNF-alpha in TGF-beta production. Also, exogenous TNF-alpha induced in a dose-dependent fashion microglial cell expression of TGF-beta 1 mRNA and release of TGF-beta. Exogenous TGF-beta, on the other hand, suppressed LPS-stimulated TNF-alpha release. These findings suggest an autoregulation of microglial cell TNF-alpha production by TGF-beta which may limit inflammation-associated brain injury.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Pentoxifylline,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0090-1229
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
358-65
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7586747-Animals,
pubmed-meshheading:7586747-Animals, Newborn,
pubmed-meshheading:7586747-Antibodies, Monoclonal,
pubmed-meshheading:7586747-Base Sequence,
pubmed-meshheading:7586747-Cells, Cultured,
pubmed-meshheading:7586747-DNA Primers,
pubmed-meshheading:7586747-DNA Probes,
pubmed-meshheading:7586747-Lipopolysaccharides,
pubmed-meshheading:7586747-Mice,
pubmed-meshheading:7586747-Mice, Inbred BALB C,
pubmed-meshheading:7586747-Microglia,
pubmed-meshheading:7586747-Molecular Sequence Data,
pubmed-meshheading:7586747-Pentoxifylline,
pubmed-meshheading:7586747-Phosphodiesterase Inhibitors,
pubmed-meshheading:7586747-Polymerase Chain Reaction,
pubmed-meshheading:7586747-RNA, Messenger,
pubmed-meshheading:7586747-Transforming Growth Factor beta,
pubmed-meshheading:7586747-Tumor Necrosis Factor-alpha
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pubmed:year |
1995
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pubmed:articleTitle |
Tumor necrosis factor-alpha mediates the release of bioactive transforming growth factor-beta in murine microglial cell cultures.
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pubmed:affiliation |
Minneapolis Medical Research Foundation, Department of Medicine, Hennepin County Medical Center, Minnesota, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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