Linked Life Data

7539221

Source:http://linkedlifedata.com/resource/pubmed/id/7539221

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5 Pt 1
pubmed:dateCreated
1995-6-29
pubmed:abstractText
To investigate the role of laminin (Ln) and merosin (a Ln variant) in the modulation of intestinal epithelial cell differentiation, we have analyzed their functional expression as well as their potential influence during the differentiation process of Caco-2 cells, a human cell line unique in its property to differentiate into a mature enterocyte-like cell type in vitro. By indirect immunofluorescence and Western blotting, Caco-2 cells have been found to express the B1 and B2 chains of Ln, as well as a heavy approximately 350- to 370-kDa chain related to the human A chain, but not the M chain, of merosin. A gradual deposition of this A-like chain-containing Ln molecule has been observed as Caco-2 cells undergo differentiation. At the cellular level, a clear relationship between basal staining for the A chain and apical staining for the brush-border membrane enzyme sucrase-isomaltase (SI) has been observed. Human Ln, used as substratum, has been found to increase significantly the expression of SI and lactase in Caco-2 cells, whereas both Ln and human merosin have been shown to stimulate aminopeptidase N and alkaline phosphatase expression. Taken together, these data indicate that enterocytic differentiation-related gene expression is promoted by an extracellular deposition of Ln and may be susceptible to a differential modulation by variant forms of the molecule.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
268
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G857-67
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Extracellular heterotrimeric laminin promotes differentiation in human enterocytes.
pubmed:affiliation
Département d'Anatomie et de Biologie Cellulaire, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't