Linked Life Data

7537088

Source:http://linkedlifedata.com/resource/pubmed/id/7537088

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1995-6-1
pubmed:abstractText
1H NMR has been used to investigate the structural properties of RANTES, a protein from the C-C branch of the chemotactic cytokine family that has a strong chemoattractive effect on monocytes, lymphocytes, and eosinophils. Titration of pH from 5.0 to 2.5 indicates that RANTES is extensively aggregated in solution above pH 4.0. At pH 3.7 the protein is mostly dimeric, although this species does dissociate to the monomer with a Kd of 35 microM. NMR data have been acquired and resonance assignments made for the dimeric species. Structures of the dimer have been generated by distance geometry and simulated annealing calculations that utilized 1956 intramolecular distance restraints, 120 intermolecular distance restraints, 164 dihedral angle restraints, and 68 restraints enforcing 34 hydrogen bonds (17.0 restraints per residue). The structure is well-defined (average root mean square deviation from the average structure of 0.38 +/- 0.06 and 0.53 +/- 0.12 A for backbone heavy atoms of residues 4-66 of the monomer and dimer, respectively). Each monomer consists of a C-terminal alpha-helix packing against a three-stranded antiparallel beta-sheet and two short N-terminal beta-strands; dimerization occurs between the N-terminal regions of each monomer. This quaternary structure is very different from that of the C-X-C chemokines such as interleukin-8 and melanoma growth stimulatory activity but similar to that found for the C-C chemokine macrophage inflammatory factor 1 beta. Distinct structural differences between RANTES and other chemokines at both the tertiary and quaternary level are discussed with regard to the distinct biological functions of the C-C and C-X-C members of this protein family.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5329-42
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:7537088-Amino Acid Sequence, pubmed-meshheading:7537088-Animals, pubmed-meshheading:7537088-Chemokine CCL4, pubmed-meshheading:7537088-Chemokine CCL5, pubmed-meshheading:7537088-Chromatography, Gel, pubmed-meshheading:7537088-Cloning, Molecular, pubmed-meshheading:7537088-Cytokines, pubmed-meshheading:7537088-Eosinophils, pubmed-meshheading:7537088-Escherichia coli, pubmed-meshheading:7537088-Hydrogen Bonding, pubmed-meshheading:7537088-Interleukin-8, pubmed-meshheading:7537088-Light, pubmed-meshheading:7537088-Lymphocytes, pubmed-meshheading:7537088-Lymphokines, pubmed-meshheading:7537088-Macrophage Inflammatory Proteins, pubmed-meshheading:7537088-Magnetic Resonance Spectroscopy, pubmed-meshheading:7537088-Models, Molecular, pubmed-meshheading:7537088-Molecular Sequence Data, pubmed-meshheading:7537088-Monocytes, pubmed-meshheading:7537088-Monokines, pubmed-meshheading:7537088-Protein Conformation, pubmed-meshheading:7537088-Protein Structure, Secondary, pubmed-meshheading:7537088-Recombinant Proteins, pubmed-meshheading:7537088-Scattering, Radiation, pubmed-meshheading:7537088-Sequence Homology, Amino Acid
pubmed:year
1995
pubmed:articleTitle
Proton NMR assignments and solution conformation of RANTES, a chemokine of the C-C type.
pubmed:affiliation
Department of Protein Engineering, Genetech, Inc., South San Francisco, California 94080, USA.
pubmed:publicationType
Journal Article, Comparative Study