Linked Life Data

7518062

Source:http://linkedlifedata.com/resource/pubmed/id/7518062

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1994-8-8
pubmed:abstractText
In this study, we show that the essential nutrient pyrroloquinoline quinone (PQQ; 50 microM) regulates N-methyl-D-aspartate (NMDA; 10 microM) receptor activity primarily by reversing the increase in the frequency of openings of the receptor-associated ion channel after chemical reduction with dithiothreitol (DTT; 1 mM). Similar to other redox-active agents, PQQ (50-200 microM) had no effect on the single-channel conductance or arithmetic mean open time of NMDA-activated events. In other experiments, we observed that inhibitory effects of PQQ (50 microM) on NMDA (30 microM)-induced whole-cell responses could be abolished by prior N-ethylmaleimide (500 microM) alkylation of the putative thiol residues that likely comprise the redox site of the receptor. These results demonstrate that PQQ modulates the NMDA receptor by directly oxidizing its redox modulatory site.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0304-3940
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
168
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
189-92
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Further evidence that pyrroloquinoline quinone interacts with the N-methyl-D-aspartate receptor redox site in rat cortical neurons in vitro.
pubmed:affiliation
Department of Neurobiology, University of Pittsburgh School of Medicine, PA 15261.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.