|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1994-2-22
|
|
pubmed:abstractText |
We recently developed a two-step methyl cellulose culture system for murine lymphohemopoietic progenitors that are capable of differentiation along the myeloid and B-lymphoid lineages. In this system, two-factor combinations, which include steel factor plus interleukin (IL) 6, IL-11, or granulocyte colony-stimulating factor effectively supported the lymphomyeloid potential of primary colonies. Interestingly, IL-3 could neither replace nor act synergistically with steel factor in maintaining the B-lymphoid potential of the primary colonies although the frequency of colony formation was the same with IL-3 and steel factor. We now report that addition of IL-3 or IL-1 alpha to a permissive system suppresses the B-lymphoid potential of primitive progenitor cells in primary culture in dose-dependent fashion. In vivo transfer of the primary colonies to scid mice confirmed the suppressive effects of IL-3 and IL-1 alpha. In addition, IL-1 alpha inhibited pre-B-cell colony formation in the secondary culture. Once pre-B-cell colonies had formed in secondary culture, neither factor affected the proliferation of the pre-B cells. These results suggest negative regulatory roles for IL-3 and IL-1 alpha in early stages of B lymphopoiesis.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-1348572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-1372992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-1373892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-1375116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-1564955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-1631072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-1692490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-1715219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-1825140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-1832057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-2453060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-2487673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-2783322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-2783947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-2786878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-2804342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3053902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3257991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3260256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3286769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3298286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3496134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3498944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3501121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3501371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3528176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3924409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3930522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-3933007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-6201749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507246-8499622
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
18
|
|
pubmed:volume |
91
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
469-73
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:7507246-Animals,
pubmed-meshheading:7507246-B-Lymphocytes,
pubmed-meshheading:7507246-Cell Division,
pubmed-meshheading:7507246-Cells, Cultured,
pubmed-meshheading:7507246-Cytokines,
pubmed-meshheading:7507246-Hematopoiesis,
pubmed-meshheading:7507246-Hematopoietic Cell Growth Factors,
pubmed-meshheading:7507246-Hematopoietic Stem Cells,
pubmed-meshheading:7507246-Interleukin-1,
pubmed-meshheading:7507246-Interleukin-3,
pubmed-meshheading:7507246-Kinetics,
pubmed-meshheading:7507246-Mice,
pubmed-meshheading:7507246-Mice, SCID,
pubmed-meshheading:7507246-Stem Cell Factor
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Negative regulation of early B lymphopoiesis by interleukin 3 and interleukin 1 alpha.
|
|
pubmed:affiliation |
Department of Medicine, Medical University of South Carolina, Charleston.
|
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
