Linked Life Data

7384194

Source:http://linkedlifedata.com/resource/pubmed/id/7384194

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1980-8-15
pubmed:abstractText
The binding of reactive benzo[a]pyrene metabolites to deproteinized DNA in vitro can be drastically changed, both quantitatively and qualitatively, in vitro by changes in the substrate concentration or the substrate/P-450 ratio, and in the intact animal by starvation or substitution of a 'purified protein test diet' for the regular laboratory chow. Liver, lung, and bowel microsomes from C57BL/6N and DBA/2N mice were examined. These data demonstrate the importance of dietary contaminants or nutrition during benzo[a]pyrene tumorigenesis. The profile of DNA binding of benzo[a]pyrene metabolites is also shown to be an extremely sensitive test for detecting minute amounts of induced cytochrome P1-450 and its associated aryl hydrocarbon (benzo[a]pyrene) hydroxylase (EC 1.14.14.2) activity. A direct correlation is not necessarily observed, however, between 'aryl hydrocarbon hydroxylase activity' and the amount of benzo[a]pyrene metabolites bound covalently to DNA. 'Untreated' genetically responsive mice are shown to have greater 'control' levels of benzo[a]pyrene metabolism in their various tissues than genetically nonresponsive mice simply on the basis that responsive mice have a lower threshold for 'responsiveness' to exogenous inducers inhaled and/or ingested in their crude diet, i.e., responsive 'control' animals already have partially induced enzymes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0031-7012
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-48
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
DNA binding of benzo[a]pyrene metabolites. Effects of substrate and microsomal protein concentration in vitro, dietary contaminants, and tissue differences.
pubmed:publicationType
Journal Article