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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1980-8-25
|
| pubmed:abstractText |
Hydrophobic chromatography was investigated as a purification procedure for human liver hexosaminidases. Both phenyl-Sepharose and valine-Sepharose have a high binding capacity of hexosaminidases. A degree of resolution between the A and B ioszymes is achieved with phenyl-Sepharose. Both hydrophobic supports must be used close to their capacity in order to recover the applied enzyme. Two purification procedures for human liver hexosaminidase B were employed, which resulted in recoveries of approximately 48 and 24% with final specific activities of 33,400 and 4840 nmole/min.mg, respectively.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0021-9673
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
4
|
| pubmed:volume |
190
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
385-91
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading | |
| pubmed:year |
1980
|
| pubmed:articleTitle |
High-capacity method for purification of human liver hexosaminidase B using hydrophobic chromatography.
|
| pubmed:publicationType |
Journal Article
|