Linked Life Data

7146087

Source:http://linkedlifedata.com/resource/pubmed/id/7146087

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1983-1-19
pubmed:abstractText
In an earlier study, we demonstrated that human exposure to ethylene oxide (EO) during sterilization procedures was associated with increased sister chromatid exchanges (SCE) in cultured peripheral blood. To study further toxic and mutagenic properties of EO in vitro, we devised a membrane dosimetry system which can be used to expose lymphocytes in peripheral blood to specified amounts of EO. The system consists of an exposure chamber, cell culture dishes to which are applied plastic membranes of varying diameters and an infrared analyzer used to monitor ambient EO concentrations in the system. Aliquots of EO-exposed media were subjected to gas chromatography for quantitation. Our preliminary analyses of the cultured cells indicate that the membrane dosimetry system we developed is capable of reproducibly delivering quantities of EO to target cells. Elevated SCEs were observed at as little as 10 micrograms/ml (in media) during a 20-min exposure period. A significant dose-response relationship between SCE and EO dose was recorded up to 35 micrograms/ml, the highest dose tested. Lymphocytes at 35 micrograms/ml showed a fourfold increase in SCE compared to control cultures. We conclude that the membrane dosimetry system provides a reproducible method to investigate EO mutagenesis in human cells in vitro and may prove applicable to a wide range of mutagenic gases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0031-7012
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
214-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Ethylene oxide induced sister chromatid exchange in human lymphocytes using a membrane dosimetry system.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't