Linked Life Data

6833711

Source:http://linkedlifedata.com/resource/pubmed/id/6833711

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-5-27
pubmed:abstractText
The kinetics of agglomeration of proteins precipitating in a viscous solution was measured by light scattering. The resulting transitory maximum was linearly proportional to the mass of protein over three orders of magnitude. The change in scattering intensity is described as a change in scattering symmetry due to a continuous increase in particle size. This method is both fast (minutes) and sensitive (30 ng protein) and is independent of the chemical composition of the different protein species and is barely influenced by size and shape of the proteins. By solubilising the protein samples in an alkaline dodecyl sulfate solution this method can be applied to all types of biological samples (e.g. tissue homogenates, membrane proteins) and also to all types of biological preparations containing detergents as well as urea, sucrose, salts and lipids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0165-022X
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-60
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Kinetics of protein agglomeration. A nephelometric method for the determination of total protein in biological samples.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't