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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1982-1-9
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pubmed:abstractText |
The presence of laminin in authentic basement membranes was examined at the level of a large pepsin-resistant fragment P1. This strongly antigenic fragment has been recently isolated from a mouse tumour basement membrane. By using antibodies to mouse laminin P1 for identification it was possible to isolate a homologous fragment P1 (Mr about 250 000) and a related component Pa (Mr about 70 000--90 000) from pepsin digests of human placenta and kidney. The fragments were in half-cystine (90--130 residues/1000) and carbohydrate and showed strong binding to concanavalin A. Reduction of disulphide bonds produced several smaller peptide chains, indicating a complex pepsin cleavage. Immunological assays demonstrated partial antigenic identity between laminin fragments obtained from mouse and human tissue, and suggested that fragment Pa may originate from a protein not completely identical with laminin. The results showed that laminin is an abundant component of tissue rich in basement membranes, which has been previously suggested by immunohistological studies.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-114518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-13894551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-226531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-371988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-387884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-391564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-4198817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-4326447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-456357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-4614671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-465457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-562261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-6250829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-6250836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-6767138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-6778807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-6987652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-6988303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-6991121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-6991310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-6995470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-88263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-884846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6796051-911892
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
193
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
749-55
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6796051-Amino Acids,
pubmed-meshheading:6796051-Basement Membrane,
pubmed-meshheading:6796051-Cross Reactions,
pubmed-meshheading:6796051-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6796051-Female,
pubmed-meshheading:6796051-Glycoproteins,
pubmed-meshheading:6796051-Humans,
pubmed-meshheading:6796051-Kidney,
pubmed-meshheading:6796051-Laminin,
pubmed-meshheading:6796051-Membrane Proteins,
pubmed-meshheading:6796051-Pepsin A,
pubmed-meshheading:6796051-Peptide Fragments,
pubmed-meshheading:6796051-Placenta,
pubmed-meshheading:6796051-Pregnancy,
pubmed-meshheading:6796051-Radioimmunoassay
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pubmed:year |
1981
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pubmed:articleTitle |
Isolation and characterization of pepsin fragments of laminin from human placental and renal basement membranes.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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