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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1981-7-20
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pubmed:abstractText |
The capsular-like envelope of Legionella pneumophila strains Togus 1 (serotype 2) and Philadelphia 1 (serotype 1) was isolated and purified by column chromatography on Sepharose 6B. Antibody raised in rabbits to these two antigenic materials did not cross-react in gel diffusion. Upon electrophoresis followed by gel diffusion, the majority of both envelope materials was found to migrate towards the cathode. A minor antigenic component of each envelope only migrated slightly towards the anode. Using the envelope antigens and the two anti-envelope sera in a counterimmunoelectrophoresis (CIE) assay, positive results were only obtained when the antigenic materials were placed in the cathodal well. The Togus 1 and Philadelphia 1 antigens did not cross-react in CIE. The sensitivity of the CIE assay was poor (15.6 micrograms/ml by carbohydrate content) compared to its sensitivity in other microbial systems. Although CIE may not be a useful diagnostic aid in identifying Legionella species due to its low sensitivity, it may be of value in serotyping the microorganism since we did not see cross-reactivity between the two strains when anti-envelope sera were used.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-2621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-286812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-304861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-335244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-335245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-357440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-359594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-383744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-386497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-400818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-434650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-434657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-4797918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-528786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-650649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-66299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-6986404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-7012173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-7354129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6785310-86313
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
637-42
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1981
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pubmed:articleTitle |
Detection of Legionella pneumophila capsular-like envelope antigens by counterimmunoelectrophoresis.
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pubmed:publicationType |
Journal Article
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