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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1980-6-25
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pubmed:abstractText |
Unintegrated viral DNA was isolated via the Hirt procedure from mouse fibroblasts newly infected with Friend murine leukemia virus (F-MuLV) clone 201, a biologically cloned helper virus isolated from stocks of F-MuLV complex. A physical map of the unintegrated in vivo linear viral DNA was generated for several restriction endonucleases. The supercoiled viral DNA was digested with EcoRI, which cleaved the viral DNA at a unique site. The linearized viral DNA was then inserted into lambda gtWES.lambda B at the EcoRI site and cloned in an approved EK2 host. Eight independent lambda-mouse recombinants were identified as containing F-MuLV DNA inserts by hybridization with F-MuLV 32P-labeled complementary DNA. One of the F-MuLV DNA inserts was 9.1 kilobases (kb) and had the same restriction enzyme sites as the unintegrated linear F-MuLV DNA. Six inserts were 8.5 kb; each lacked a single copy of the terminally redundant sequences of the unintegrated linear viral DNA. One insert was 8.2 kb and contained a 0.9-kb deletion. After digestion with EcoRI, one recombinant DNA preparation containing an 8.5-kb insert was infectious for NIH 3T3 cells. Undigested recombinant DNA was not infectious. The infectivity of the EcoRI-digested DNA followed multihit kinetics, indicating that more than one molecule was required to register as an infectious unit. The virus isolated from this transfection (F-MuLV-57) was NB-ecotropic, helper-independent, and formed XC plaques. Inoculation of this virus into newborn NIH Swiss mice induced leukemia and splenomegaly in greater than 90% of animals within 3 to 4 weeks. The gross and microscopic abnormalities induced by F-MuLV clone 57 were identical to those seen with the original parent stocks of F-MuLV clone 201. These results indicate that this helper-independent F-MuLV can induce a rapid nonthymic leukemia in the absence of the spleen focus-forming virus.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-1061080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-13416470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-13618884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-163329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-170411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-183818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-190768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-206001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-215324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-215781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-26810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-322278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-338419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-366607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-4291934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-4308055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-4311790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-4350228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-455438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-655381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-68125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-686360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245244-88264
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
475-86
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6245244-Animals,
pubmed-meshheading:6245244-Bacteriophage lambda,
pubmed-meshheading:6245244-Cell Line,
pubmed-meshheading:6245244-Chromosome Mapping,
pubmed-meshheading:6245244-Cloning, Molecular,
pubmed-meshheading:6245244-DNA, Recombinant,
pubmed-meshheading:6245244-DNA, Viral,
pubmed-meshheading:6245244-Fibroblasts,
pubmed-meshheading:6245244-Friend murine leukemia virus,
pubmed-meshheading:6245244-Mice,
pubmed-meshheading:6245244-Mice, Inbred Strains,
pubmed-meshheading:6245244-Retroviridae,
pubmed-meshheading:6245244-Transfection
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pubmed:year |
1980
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pubmed:articleTitle |
Transfection of molecularly cloned Friend murine leukemia virus DNA yields a highly leukemogenic helper-independent type C virus.
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pubmed:publicationType |
Journal Article
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