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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1983-6-10
|
| pubmed:abstractText |
Small aliquots of DNA-containing samples from gradient or column fractions are spotted onto agarose plates containing ethidium bromide, and the DNA is visualized qualitatively. After it is overlaid with a thin agarose cover, the DNA is denatured and transferred to nitrocellulose filters for hybridization. This method, called the "spot-blot assay," is particularly convenient when dealing with large numbers of samples.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0003-2697
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
128
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
415-21
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6189420-Base Sequence,
pubmed-meshheading:6189420-DNA,
pubmed-meshheading:6189420-Escherichia coli,
pubmed-meshheading:6189420-Ethidium,
pubmed-meshheading:6189420-Methods,
pubmed-meshheading:6189420-Nucleic Acid Hybridization,
pubmed-meshheading:6189420-Physarum,
pubmed-meshheading:6189420-RNA
|
| pubmed:year |
1983
|
| pubmed:articleTitle |
Spot blot: a hybridization assay for specific DNA sequences in multiple samples.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|