Linked Life Data

3762739

Source:http://linkedlifedata.com/resource/pubmed/id/3762739

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-10-30
pubmed:abstractText
The uptake of 3H-noradrenaline into reserpine-pretreated PC-12 cells (a clonal cell line which possesses "uptake1") was abolished when at high extracellular Cl- all the extracellular Na+ was replaced by Tris+ and when at high extracellular Na+ all the extracellular Cl- was replaced by isethionate. Increases in the external Cl- concentration (at a fixed high Na+ concentration) progressively increased the uptake of 3H-noradrenaline. The same was found with increase in the external Na+ concentration (at a fixed high Cl- concentration). From the anions tested only Br- and SCN- were able to partially mimic the transport-stimulating effect of Cl- (with about 40% and 20% effectiveness, respectively). When chloride was replaced by nitrate or larger anions such as sulphate, methylsulphate or isethionate, virtually no transport of 3H-noradrenaline was observed. The initial rate of uptake of 3H-noradrenaline showed saturation with increasing concentrations of noradrenaline when determined at several fixed concentrations of either Na+ or Cl-. The apparent Km for noradrenaline transport (KmNA) progressively decreased and the VmaxNA increased with increases in the concentration of Na+ (at a high concentration of Cl-) or Cl- (at a high concentration of Na+). The stimulation of the initial rate of uptake of 3H-noradrenaline by increasing concentrations of either Na+ or Cl- obeyed saturation kinetics when determined at several concentrations of noradrenaline. The concentration of Na+ (or Cl-) which caused half-maximal stimulation of uptake (i.e., the apparent KmNa+ and the apparent KmCl-) decreased with increases in the concentration of noradrenaline. These results strongly suggest that Na+ and Cl- are co-transported with noradrenaline.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0028-1298
pubmed:author
pubmed:issnType
Print
pubmed:volume
333
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
246-52
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
The neuronal noradrenaline transport system of PC-12 cells: kinetic analysis of the interaction between noradrenaline, Na+ and Cl- in transport.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't