Linked Life Data

3002635

Source:http://linkedlifedata.com/resource/pubmed/id/3002635

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-3-14
pubmed:abstractText
The in vivo distribution of topoisomerase I on specific DNA sequences is determined at high resolution in Drosophila cells using a photo-crosslinking method. Topoisomerase I-DNA adducts are generated by irradiation of intact cells with UV light and then purified by immunoprecipitation with antibody to topoisomerase I. Analyses of the DNA sequences crosslinked to topoisomerase I by blot-hybridization with appropriate DNA probes indicate that topoisomerase I is concentrated on transcribed regions and not on nontranscribed flanking sequences. Like RNA polymerase II, topoisomerase I is recruited to heat-shock genes during the heat-shock response. However, topoisomerase I and RNA polymerase II can interact independently with the transcribed region because different ratios of topoisomerase I and RNA polymerase II are crosslinked to the highly transcribed hsp70 gene and the moderately transcribed copia genes. We hypothesize that topoisomerase I allows topological changes in DNA that are required for transcription.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
401-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Topoisomerase I interacts with transcribed regions in Drosophila cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't