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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1987-1-7
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pubmed:abstractText |
Human placental microsomal aminopeptidase (microsomal PAP) was purified 3,880-fold from human placenta and characterized. The enzyme was solubilized from membrane fractions with Triton X-100 and also trypsin digestion, and subjected to zinc sulfate fractionation, chromatographies with DE-52, hydroxylapatite, Sephacryl S-300 and lentil lectin-Sepharose 4B, and finally affinity chromatography with bestatin-Sepharose 4B. Microsomal PAP was separated from aminopeptidase A (AAP) by affinity chromatography. The apparent relative molecular mass (Mr) of the enzyme was estimated to be 220,000 by high-performance liquid chromatography with an aqueous gel column. The purified enzyme gave almost a single band with a molecular mass of 140,000 by sodium dodecyl sulfate (SDS) gel electrophoresis. The isoelectric point of the enzyme was 5.2. The purified enzyme was most active at pH 8.0 with L-leucine-p-nitroanilide as substrate; the Km value for this substrate was 1.1 mmol/l. The microsomal PAP was immunologically different from the pregnancy serum cystyl aminopeptidase (serum PAP).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0013-9432
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
197-205
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2877870-Aminopeptidases,
pubmed-meshheading:2877870-Antigens, CD13,
pubmed-meshheading:2877870-Binding, Competitive,
pubmed-meshheading:2877870-Chromatography, Affinity,
pubmed-meshheading:2877870-Chromatography, Gel,
pubmed-meshheading:2877870-Cystinyl Aminopeptidase,
pubmed-meshheading:2877870-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2877870-Female,
pubmed-meshheading:2877870-Humans,
pubmed-meshheading:2877870-Isoelectric Focusing,
pubmed-meshheading:2877870-Kinetics,
pubmed-meshheading:2877870-Molecular Weight,
pubmed-meshheading:2877870-Placenta,
pubmed-meshheading:2877870-Pregnancy,
pubmed-meshheading:2877870-Radioimmunoassay,
pubmed-meshheading:2877870-Substrate Specificity
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pubmed:year |
1986
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pubmed:articleTitle |
Purification and characterization of human placental microsomal aminopeptidase: immunological difference between placental microsomal aminopeptidase and pregnancy serum cystyl-aminopeptidase.
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pubmed:publicationType |
Journal Article,
Comparative Study
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