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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1988-5-12
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pubmed:abstractText |
Macrophage colony-stimulating factor (CSF-1; M-CSF) is a growth factor required for growth and differentiation of mononuclear phagocytes. The effects of CSF-1 are mediated through binding to specific, high-affinity surface receptors encoded by the c-fms gene. CSF-1 and c-fms gene expression was investigated in fresh human acute myeloblastic leukemic cells by Northern blot hybridization using cDNA probes. 4.0-kb CSF-1 transcripts were detected in 10 of 17 cases of acute myeloblastic leukemia (AML), while c-fms transcripts were detected in 7 of 15. Coexpression of CSF-1 and c-fms was observed in five cases, and in five other cases neither gene was expressed. In situ hybridization demonstrated that transcripts for CSF-1 were present in 70-90% of cells in each of three cases studied while c-fms mRNA was detected in 40-70% of cells. The constitutive expression of CSF-1 transcripts was associated with production of CSF-1 protein, although detectable amounts of CSF-1 were not secreted unless the cells were exposed to phorbol ester. These results demonstrate that leukemic myeloblasts from a subset of patients with AML express transcripts for both the CSF-1 and CSF-1 receptor genes, often in the same leukemic cells in vitro.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-2408759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-2409450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-2420874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-2434085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-2444833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-2578168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-3024013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-3027579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-3030467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-3039346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-3105621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-3488774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-3491836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-3535923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-3547079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-3698101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-3862359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-3878229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-6600723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-6945311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-6973347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832442-6978185
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Colony-Stimulating Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Colony-Stimulating Factor
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1030-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2832442-Colony-Stimulating Factors,
pubmed-meshheading:2832442-Gene Expression Regulation,
pubmed-meshheading:2832442-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:2832442-Growth Substances,
pubmed-meshheading:2832442-Humans,
pubmed-meshheading:2832442-Leukemia, Myeloid, Acute,
pubmed-meshheading:2832442-Nucleic Acid Hybridization,
pubmed-meshheading:2832442-Proto-Oncogene Proteins,
pubmed-meshheading:2832442-RNA, Messenger,
pubmed-meshheading:2832442-RNA, Neoplasm,
pubmed-meshheading:2832442-Receptors, Cell Surface,
pubmed-meshheading:2832442-Receptors, Colony-Stimulating Factor,
pubmed-meshheading:2832442-Transcription, Genetic
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pubmed:year |
1988
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pubmed:articleTitle |
Expression of the macrophage colony-stimulating factor and c-fms genes in human acute myeloblastic leukemia cells.
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pubmed:affiliation |
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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