Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1987-12-30
pubmed:abstractText
The regulatory action of activators for protein kinase C on the specific binding capacity for recombinant human tumor necrosis factor alpha (TNF-alpha) was studied on various human cell lines. Phorbol myristate acetate (PMA) and oleyl acetyl glycerol (OAG) both are able to rapidly downregulate TNF-binding capacity of normal and malignant cells derived from various tissues. As PMA treatment did not enhance internalization of TNF-alpha-receptor complexes at 37 degrees C, and since OAG was able to downregulate TNF-binding capacity under conditions where internalization and shedding of receptor protein are prevented, we conclude that protein kinase C controls ligand affinity of the TNF-receptor protein, possibly via direct phosphorylation. Protein kinase C triggered downregulation of TNF-alpha-binding capacity concomitantly resulted in reduction of TNF-alpha sensitivity, as revealed from decreased cytotoxic action of TNF-alpha on L 929 cells and from inhibition of TNF-alpha-mediated enhancement of HLA class II antigen expression in Colo 205 cells. Restoration of TNF-binding capacity upon abrogation of protein kinase C stimulation leads to full recovery of TNF responsiveness, further supporting the close linkage of TNF-receptor expression and TNF sensitivity. These data suggest that regulation of TNF-binding capacity by protein kinase C is one of the cellular control mechanisms of TNF responsiveness.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-221835, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-2433337, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-2948902, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-2993471, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-2994048, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-2999773, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3001529, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3026394, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3026955, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3029221, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3029503, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3030559, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3031163, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3033642, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3038218, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3087891, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3878923, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3933111, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-3933986, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-6238627, http://linkedlifedata.com/resource/pubmed/commentcorrection/2824656-6427923
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
166
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1788-97
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Downregulation of tumor necrosis factor (TNF) sensitivity via modulation of TNF binding capacity by protein kinase C activators.
pubmed:affiliation
Klinische Arbeitsgruppe BRWTI, Max-Planck-Gesellschaft, Göttingen, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't