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rdf:type |
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lifeskim:mentions |
umls-concept:C0009968,
umls-concept:C0025545,
umls-concept:C0040649,
umls-concept:C0043393,
umls-concept:C0205263,
umls-concept:C0815047,
umls-concept:C1413931,
umls-concept:C1522424,
umls-concept:C1555707,
umls-concept:C1705851,
umls-concept:C2752151,
umls-concept:C2828366
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pubmed:issue |
21
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pubmed:dateCreated |
1989-12-8
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pubmed:abstractText |
We describe a cell-free system in which the transcription of the yeast metallothionein gene is inducible by the addition of metal ions plus a specific regulatory protein. Efficient transcription requires the complete yeast ACE1 metalloregulatory protein, including both its DNA-binding and transactivation domains; a mouse nuclear extract providing RNA polymerase and general transcription factors; a template containing the ACE1 binding site; and Cu(I). Because the binding of ACE1 to DNA is dependent on Cu, it is possible to inhibit transcription by the use of Cu-complexing agents such as CN-. We have used this specific inhibition to show that the ACE1 regulatory protein is required for the maintenance as well as the formation of a functional preinitiation complex. The ability to reversibly induce yeast metallothionein gene transcription in vitro provides a powerful system for determining the molecular mechanism of a simple eukaryotic regulatory circuit.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-2409080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-2643107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-2651899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-2653812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-2725504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-2830021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-2830022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-2882858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3043194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3043665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3044607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3050531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3052856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3128739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3275668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3283130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3288540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3327472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3416354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3416355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3530495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3537699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3887570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-3902832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-6095291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-6257399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-6364141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-6374656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-6537904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682650-7133118
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8377-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2682650-Animals,
pubmed-meshheading:2682650-Cell Nucleus,
pubmed-meshheading:2682650-Cell-Free System,
pubmed-meshheading:2682650-Copper,
pubmed-meshheading:2682650-DNA-Binding Proteins,
pubmed-meshheading:2682650-Fungal Proteins,
pubmed-meshheading:2682650-Gene Expression Regulation, Fungal,
pubmed-meshheading:2682650-Genes, Fungal,
pubmed-meshheading:2682650-Kinetics,
pubmed-meshheading:2682650-L Cells (Cell Line),
pubmed-meshheading:2682650-Metallothionein,
pubmed-meshheading:2682650-Metals,
pubmed-meshheading:2682650-Mice,
pubmed-meshheading:2682650-Promoter Regions, Genetic,
pubmed-meshheading:2682650-Saccharomyces cerevisiae,
pubmed-meshheading:2682650-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:2682650-Templates, Genetic,
pubmed-meshheading:2682650-Transcription, Genetic,
pubmed-meshheading:2682650-Transcription Factors
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pubmed:year |
1989
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pubmed:articleTitle |
Copper and the ACE1 regulatory protein reversibly induce yeast metallothionein gene transcription in a mouse extract.
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pubmed:affiliation |
Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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