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pubmed-article:2612384pubmed:abstractTextThe examination of pair-rule gene expression in wild-type and segmentation mutant embryos has identified many, but not necessarily all, of the elements of the regulatory system that establish their periodic patterns. Here we have conducted a new type of search for previously unknown regulators of these genes by examining pair-rule gene expression in blastoderm embryos lacking parts of or entire chromosomes. This method has the advantage of direct inspection of abnormal pair-rule gene patterns without relying upon mutagenesis or interpretation of larval phenotypes for the identification of segmentation genes. From these experiments we conclude that: (i) most zygotically required regulators of the fushi tarazu (ftz), even-skipped (eve) and hairy (h) pair-rule genes have been identified, except for one or more loci we have uncovered on chromosome arm 2L; (ii) the repression of the ftz and eve genes in the anterior third of the embryo is under maternal, not zygotic control; and (iii) there are no general zygotically required activators of pair-rule gene expression. The results suggest that the molecular basis of pair-rule gene regulation can be pursued with greater confidence now that most key trans-acting factors are already in hand.lld:pubmed
pubmed-article:2612384pubmed:languageenglld:pubmed
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pubmed-article:2612384pubmed:monthNovlld:pubmed
pubmed-article:2612384pubmed:issn0950-1991lld:pubmed
pubmed-article:2612384pubmed:authorpubmed-author:CarrollS BSBlld:pubmed
pubmed-article:2612384pubmed:authorpubmed-author:VavraS HSHlld:pubmed
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pubmed-article:2612384pubmed:volume107lld:pubmed
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pubmed-article:2612384pubmed:pagination663-72lld:pubmed
pubmed-article:2612384pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:2612384pubmed:year1989lld:pubmed
pubmed-article:2612384pubmed:articleTitleThe zygotic control of Drosophila pair-rule gene expression. I. A search for new pair-rule regulatory loci.lld:pubmed
pubmed-article:2612384pubmed:affiliationLaboratory of Molecular Biology, University of Wisconsin, Madison 53706.lld:pubmed
pubmed-article:2612384pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2612384pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:2612384pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
pubmed-article:2612384pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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