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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0009015,
umls-concept:C0017262,
umls-concept:C0032200,
umls-concept:C0071253,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0443211,
umls-concept:C0597357,
umls-concept:C0678594,
umls-concept:C2348205,
umls-concept:C2911684
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pubmed:issue |
13
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pubmed:dateCreated |
1989-8-10
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pubmed:databankReference |
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pubmed:abstractText |
The primary structure of the human A-type receptor for platelet-derived growth factor (PDGF) has been determined. A 6.5-kilobase (kb) transcript was identified through low-stringency hybridization with a probe derived from the B-type PDGF receptor cDNA. The sequence of a cDNA clone corresponding to the 6.5-kb transcript contains an open reading frame that predicts a 1089-amino acid growth factor receptor-like molecule, which displays 44% overall amino acid similarity with the PDGF B-type receptor. The two receptors have a similar domain organization, with five immunoglobulin-like domains extracellularly and an intracellular split protein tyrosine kinase domain. Transfection of the new cDNA into COS cells led to the expression of a protein specifically recognized by an antiserum previously shown to react with the PDGF A-type receptor. The expressed protein was shown to display high-affinity binding of all three 125I-labeled dimeric forms of PDGF A and B chains in a manner that is characteristic for the PDGF A-type receptor.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-222468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2421165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2448137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2460450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2536372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2536956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2835772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2836952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2842060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2842148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2842689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2850496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2984061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-2991903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-3013421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-3020426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-3029095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-3456080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-3488815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-3497714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-3714490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-3754619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-3935400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-3943125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-4733239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-5963888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-6206062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-6273838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-6318322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-6329745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2544881-6526008
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4917-21
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2544881-Amino Acid Sequence,
pubmed-meshheading:2544881-Animals,
pubmed-meshheading:2544881-Base Sequence,
pubmed-meshheading:2544881-Cell Line,
pubmed-meshheading:2544881-Cloning, Molecular,
pubmed-meshheading:2544881-DNA,
pubmed-meshheading:2544881-Humans,
pubmed-meshheading:2544881-Molecular Sequence Data,
pubmed-meshheading:2544881-Nucleic Acid Hybridization,
pubmed-meshheading:2544881-Platelet-Derived Growth Factor,
pubmed-meshheading:2544881-Receptors, Cell Surface,
pubmed-meshheading:2544881-Receptors, Platelet-Derived Growth Factor,
pubmed-meshheading:2544881-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2544881-Structure-Activity Relationship,
pubmed-meshheading:2544881-Transfection
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pubmed:year |
1989
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pubmed:articleTitle |
cDNA cloning and expression of the human A-type platelet-derived growth factor (PDGF) receptor establishes structural similarity to the B-type PDGF receptor.
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pubmed:affiliation |
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
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pubmed:publicationType |
Journal Article,
Comparative Study
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