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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-5-26
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pubmed:abstractText |
Recombinant transforming growth factor (TGF)-beta 1 precursor was recently found to contain mannose 6-phosphate (Purchio et al., 1988, J. Biol. Chem. 263, 14211-14215). In the present study, recombinant TGF-beta 1 precursor was shown to bind to the insulin-like growth factor (IGF)-II/mannose 6-phosphate (man6P) receptor on the plasma membrane of cells since: 1) Insulin, which induces an increase in cell surface IGF-II/man6P receptors on adipocytes, caused a 2.7-fold increase in TGF-beta 1 precursor binding to adipocytes; 2) Chinese hamster ovary cells selected for overexpression of the IGF-II/man6P receptor exhibited an increased binding of TGF-beta 1 precursor in comparison to the parental cells; and 3) the binding of 125I-TGF-beta 1 precursor to these transfected cells and adipocytes was largely inhibited by man6P. After 15 minutes at 37 degrees C, 75% of the recombinant TGF-beta 1 precursor was found to be internalized in the transfected cells. Additional studies with latent TGF-beta 1 isolated from platelets indicated that this material could also bind to the isolated IGF-II/man6P receptor.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Mannosephosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, IGF Type 2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Somatomedin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/mannose-6-phosphate
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
160
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
393-403
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2540751-Adipose Tissue,
pubmed-meshheading:2540751-Animals,
pubmed-meshheading:2540751-Blood Platelets,
pubmed-meshheading:2540751-Cell Line,
pubmed-meshheading:2540751-Cell Membrane,
pubmed-meshheading:2540751-DNA,
pubmed-meshheading:2540751-Insulin,
pubmed-meshheading:2540751-Male,
pubmed-meshheading:2540751-Mannosephosphates,
pubmed-meshheading:2540751-Protein Precursors,
pubmed-meshheading:2540751-Rats,
pubmed-meshheading:2540751-Rats, Inbred Strains,
pubmed-meshheading:2540751-Receptor, IGF Type 2,
pubmed-meshheading:2540751-Receptors, Cell Surface,
pubmed-meshheading:2540751-Receptors, Somatomedin,
pubmed-meshheading:2540751-Recombinant Proteins,
pubmed-meshheading:2540751-Transfection,
pubmed-meshheading:2540751-Transforming Growth Factors
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pubmed:year |
1989
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pubmed:articleTitle |
Interactions of recombinant and platelet transforming growth factor-beta 1 precursor with the insulin-like growth factor II/mannose 6-phosphate receptor.
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pubmed:affiliation |
Department of Pharmacology, Stanford University School of Medicine, CA 94305-5332.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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