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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0003320,
umls-concept:C0007585,
umls-concept:C0014441,
umls-concept:C0054950,
umls-concept:C0229671,
umls-concept:C0391871,
umls-concept:C0431085,
umls-concept:C0728938,
umls-concept:C1456796,
umls-concept:C1511790,
umls-concept:C1515655,
umls-concept:C1533691,
umls-concept:C1550101,
umls-concept:C1705984,
umls-concept:C1749467,
umls-concept:C1880022
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pubmed:issue |
1
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pubmed:dateCreated |
1989-4-17
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pubmed:abstractText |
An enzyme-linked immunosorbent assay (ELISA) has been developed that allows the quantitative determination of the Ki-1 (CD30) antigen in soluble form. Similar levels of sensitivity of this new Ki-1 ELISA and the ELISA previously described for measuring the soluble 55-kDa chain of the interleukin 2 receptor were seen. As assessed with this ELISA, the investigated Ki-1+ permanent cell lines released the Ki-1 antigen into the culture supernatant. In culture supernatants of concanavalin A-activated human peripheral blood lymphocytes, however, this antigen could not be detected. The released Ki-1 antigen has an apparent molecular weight (Mr) of 85,000, whereas the cell-associated Ki-1 antigen has an Mr of 105,000. We investigated sera from 30 normal donors, 15 patients with systemic infections, and 63 patients suffering from lymphomas for soluble Ki-1 antigen. In all sera from normal donors and patients with systemic infectious diseases, soluble Ki-1 antigen was below the detection limit (i.e., less than 70 pg). In contrast, high amounts of the soluble Ki-1 antigen were found in sera from 18 malignant lymphomas containing Ki-1+ tumor cells. This finding demonstrates that the release of the Ki-1 antigen takes place not only in vitro, but in vivo as well. Moreover, these results imply that the Ki-1 antigen may be used as a serum tumor marker.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD30,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-2980
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
157-62
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2537734-Adult,
pubmed-meshheading:2537734-Antibodies, Monoclonal,
pubmed-meshheading:2537734-Antigens, CD30,
pubmed-meshheading:2537734-Antigens, Differentiation,
pubmed-meshheading:2537734-Antigens, Neoplasm,
pubmed-meshheading:2537734-Cell-Free System,
pubmed-meshheading:2537734-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2537734-Humans,
pubmed-meshheading:2537734-Kinetics,
pubmed-meshheading:2537734-Lymphoma,
pubmed-meshheading:2537734-Molecular Weight,
pubmed-meshheading:2537734-Receptors, Interleukin-2,
pubmed-meshheading:2537734-Subcellular Fractions,
pubmed-meshheading:2537734-Tumor Cells, Cultured
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pubmed:year |
1989
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pubmed:articleTitle |
Ki-1 (CD30) antigen is released by Ki-1-positive tumor cells in vitro and in vivo. I. Partial characterization of soluble Ki-1 antigen and detection of the antigen in cell culture supernatants and in serum by an enzyme-linked immunosorbent assay.
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pubmed:affiliation |
Institute of Immunology, Klinikum Steglitz, Free University of Berlin.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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