Linked Life Data

2477391

Source:http://linkedlifedata.com/resource/pubmed/id/2477391

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-11-20
pubmed:abstractText
A simple and rapid technique is described whereby several unfertilized oocytes and embryos can be processed simultaneously for embedding and sectioning for histological analysis. Oocytes are encased in a serum matrix and thereafter can be handled with ease. A total of 112 unfertilized oocytes and abnormal embryos following insemination in vitro have been examined. Only 31% of oocytes showed pyknotic nuclei after a mean of 4.0 +/- 1.24 days in culture and 37% had recognizable metaphase II chromosomes. Twenty per cent showed evidence of fertilization, but spermatozoa had failed entirely to penetrate the zonae pellucidae of 34% of oocytes. The mean percentages of penetrating sperm were not significantly different between oocytes which did and did not fertilize. Under these in-vitro conditions, approximately 1-2% of spermatozoa reaching the oocyte penetrated into the zona pellucida and a lesser number to the perivitelline space. Polar bodies were also observed and the incidence of divisions, retention and parthenogenesis estimated. This technique may advance our understanding of the reasons for the success or failure of fertilization in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0268-1161
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
588-94
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
A simple method for the morphological analysis of human ova: zona penetration in oocytes failing to fertilize or fertilizing abnormally in vitro.
pubmed:affiliation
Bourn Hall Clinic, Cambridge, UK.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't