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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1989-9-12
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pubmed:abstractText |
To further characterize the mechanism involved in poliovirus-induced inhibition of HeLa cells mRNA synthesis, in vitro formation of DNA-protein complexes between nuclear upstream stimulatory transcription factor (USF) and the adenovirus type 2 major late promoter upstream promoter element (UPE; located between -45 and -65 base pairs) was studied. Using the gel shift assay, we found differences between the UPE-protein complex formed with partially purified nuclear extracts from poliovirus-infected HeLa cells and that obtained in the presence of mock-infected extracts. Formation of the modified UPE-USF complex coincided with virus-induced inhibition of host cell RNA synthesis in vivo and with a less efficient in vitro transcriptional activity of the nuclear extracts from infected cells. Furthermore, using a cross-linking protocol, we found that the host 46-kilodalton UPE-binding USF factor was severely diminished and that a virus-induced or -modified 50-kilodalton polypeptide appeared to be specifically bound to the UPE template.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3858-64
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2474675-DNA,
pubmed-meshheading:2474675-DNA-Binding Proteins,
pubmed-meshheading:2474675-HeLa Cells,
pubmed-meshheading:2474675-Humans,
pubmed-meshheading:2474675-Poliovirus,
pubmed-meshheading:2474675-Promoter Regions, Genetic,
pubmed-meshheading:2474675-RNA,
pubmed-meshheading:2474675-RNA Polymerase II,
pubmed-meshheading:2474675-Transcription Factors,
pubmed-meshheading:2474675-Viral Proteins
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pubmed:year |
1989
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pubmed:articleTitle |
Modification of an adenovirus major late promoter-binding factor during poliovirus infection.
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pubmed:affiliation |
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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