Linked Life Data

2426295

Source:http://linkedlifedata.com/resource/pubmed/id/2426295

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-9-17
pubmed:abstractText
An indirect immunogold staining technique was used for identification of hepatitis A virus and poliovirus types 1,2 and 3 by electron microscopy. Human convalescent antisera against hepatitis A virus and the three poliovirus types were used as primary antibodies and goat anti-human IgG gold complexes, with an approximate gold granule size of 15 nm, as secondary antibody. Hepatitis A virus was specifically labelled with the homologous antiserum. No labelling was observed when antiserum against poliovirus or other negative control sera were used as primary antibody. Poliovirus types 1, 2 and 3 were specifically labelled with a human serum with high titres of antibody against all three virus types. The use of type-specific antisera made it possible to distinguish types 1, 2 and 3 from each other, although slight cross-reactions occurred. The results indicate that gold IgG complexes in conjunction with human antisera may be used for identification of small spherical viruses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
207-14
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Use of gold IgG complexes and human antisera for electron microscopic identification of hepatitis A virus and polioviruses.
pubmed:publicationType
Journal Article