Linked Life Data

2408622

Source:http://linkedlifedata.com/resource/pubmed/id/2408622

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1985-7-2
pubmed:abstractText
Receptor-mediated endocytosis in suspended hepatocytes was studied in conjunction with ATP levels of the cells, which were decreased by the use of metabolic inhibitors. The receptor system studied was the asialo-glycoprotein receptor, and multiple aspects of the endocytic pathway were examined: binding of ligand, internalization, intracellular transport and proteolysis. Moderate concentrations of the inhibitors (e.g. 30 microM rotenone or 200 microM iodoacetamide) produced only a transient decline in the ATP levels of the cells. Two to four times higher concentrations reduced the ATP levels to about 1/10 of control cells. At low levels of ATP (less than 30% of controls) the uptake ceased completely after 10-20 min. Moderate reductions brought about by rotenone reduced the uptake roughly in proportion to the ATP levels; iodoacetamide and sodium fluoride had little influence on the energy production by the cells, but the rate of asialo-glycoprotein uptake was reduced to a small fraction of controls. The effect of rotenone on the rate of uptake was mainly due to a lower rate of internalization of occupied receptors; the half-time for internalization of surface-bound ligand was increased from 2.9 to 6.2 min in the presence of 42 microM rotenone. The binding capacity of the cell surface was also somewhat lower. There was no degradation of the asialo-glycoproteins which were taken up by cells treated with high concentrations of rotenone or iodoacetamide. This was shown to be due to a low rate of transport of the endocytosed protein into those endosomes (at density 1.15 g/ml in a sucrose gradient) which were delivering their contents to the lysosomes; coincidentally, there was an accumulation of ligand in light endosomes (density 1.11 g/ml), in which the ligand appears immediately after endocytosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate, http://linkedlifedata.com/resource/pubmed/chemical/Asialoglycoprotein Receptor, http://linkedlifedata.com/resource/pubmed/chemical/Asialoglycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Fetuins, http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes, http://linkedlifedata.com/resource/pubmed/chemical/Iodoacetamide, http://linkedlifedata.com/resource/pubmed/chemical/Orosomucoid, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic, http://linkedlifedata.com/resource/pubmed/chemical/Rotenone, http://linkedlifedata.com/resource/pubmed/chemical/alpha-Fetoproteins, http://linkedlifedata.com/resource/pubmed/chemical/asialofetuin, http://linkedlifedata.com/resource/pubmed/chemical/asialoorosomucoid
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-2952
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1639-45
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
The influence of cellular ATP levels on receptor-mediated endocytosis and degradation of asialo-glycoproteins in suspended hepatocytes.
pubmed:publicationType
Journal Article, In Vitro