|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0031308,
umls-concept:C0044602,
umls-concept:C0079633,
umls-concept:C0162326,
umls-concept:C0185117,
umls-concept:C0285761,
umls-concept:C0332256,
umls-concept:C0597551,
umls-concept:C0851285,
umls-concept:C1150481,
umls-concept:C1335283,
umls-concept:C1368105,
umls-concept:C1451005,
umls-concept:C1705325,
umls-concept:C1705637,
umls-concept:C2911684
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2011-9-7
|
|
pubmed:abstractText |
B-cell activation factor of the tumour necrosis factor family (BAFF), an important regulator of B-cell survival, has recently been found to be expressed on the surface of murine and human macrophages and engagement with its receptor was shown to trigger induction of pro-inflammatory mediators and block phagocytic activity. In an effort to generate immunomodulatory agents that can regulate BAFF-mediated signal, decapeptides representing the intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) of immune receptor expressed on myeloid cells (IREM)-1, an inhibitory transmembrane protein expressed on myeloid cells, were synthesized in conjugation with HIV-transactivator of transcription (TAT)(48-57,) which facilitates the internalization of peptides into cells. Interestingly, all five of these synthetic peptides, representing the five ITIM-like sequences present in the cytoplasmic tail of IREM-1, exhibited inhibitory action against BAFF-mediated induction of matrix metalloproteinase-9 and interleukin-8 expression. Inhibitor assay and immunoprecipitation assay followed by Western blotting demonstrated that the inhibitory action was mediated by Src homology 2 (SH2)-containing tyrosine phosphatase (SHP)-1 and/or phosphoinositide 3-kinase (PI3K). ELISA-based nuclear factor-?B DNA binding assay observed that the synthetic peptides blocked the activation of nuclear factor-?B in an SHP-1 and phosphoinositide 3-kinase-dependent manner. Three of these synthetic peptides exhibited varying degrees of inhibitory action against BAFF-mediated blockage of phagocytosis in a SHP-1 and PI3K-dependent manner. These data indicate that the synthetic peptides are capable of blocking BAFF-mediated regulation of macrophage activities through the activation of SHP-1 and PI3K as well as inhibition of nuclear factor-?B activation.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/B-Cell Activating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/CD300LF protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Immunologic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 9,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/PTPN6 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/TNFSF13B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
1365-2567
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
© 2011 The Authors. Immunology © 2011 Blackwell Publishing Ltd.
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
134
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
224-33
|
|
pubmed:meshHeading |
pubmed-meshheading:21896016-Amino Acid Motifs,
pubmed-meshheading:21896016-Amino Acid Sequence,
pubmed-meshheading:21896016-B-Cell Activating Factor,
pubmed-meshheading:21896016-Cell Line, Tumor,
pubmed-meshheading:21896016-Humans,
pubmed-meshheading:21896016-Immunologic Factors,
pubmed-meshheading:21896016-Interleukin-8,
pubmed-meshheading:21896016-Matrix Metalloproteinase 9,
pubmed-meshheading:21896016-Molecular Sequence Data,
pubmed-meshheading:21896016-NF-kappa B,
pubmed-meshheading:21896016-Oligopeptides,
pubmed-meshheading:21896016-Phagocytosis,
pubmed-meshheading:21896016-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:21896016-Protein Tyrosine Phosphatase, Non-Receptor Type 6,
pubmed-meshheading:21896016-Receptors, Immunologic,
pubmed-meshheading:21896016-tat Gene Products, Human Immunodeficiency Virus
|
|
pubmed:year |
2011
|
|
pubmed:articleTitle |
Synthetic peptides containing ITIM-like sequences of IREM-1 inhibit BAFF-mediated regulation of interleukin-8 expression and phagocytosis through SHP-1 and/or PI3K.
|
|
pubmed:affiliation |
School of Life Sciences and Biotechnology, Kyungpook National University, Daegu, Korea.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
