Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1990-6-13
pubmed:databankReference
pubmed:abstractText
We have recently identified and characterized a 53-kDa inner nuclear membrane-associated protein in Drosophila and termed it otefin. Here we report the isolation and characterization of cDNA and genomic clones of the otefin gene. Based on sequence analysis, we deduced that the primary translation product has a calculated mass of 45 kDa, contains many serine and threonine residues, and is mostly hydrophilic. However, in the carboxyl terminus, there is a hydrophobic region which may serve as a membrane anchoring domain. RNA blot analysis indicated that the otefin gene codes for a single poly(A+) transcript of 1.6 kilobases and that relatively large amounts of this transcript are present during developmental stages in which many nuclear divisions occur. Polyclonal antibodies raised against the cDNA translation product react with a 58-kDa mammalian nuclear envelope protein, demonstrating evolutionary conservation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
265
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7808-13
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:2186029-Amino Acid Sequence, pubmed-meshheading:2186029-Animals, pubmed-meshheading:2186029-Base Sequence, pubmed-meshheading:2186029-Blotting, Western, pubmed-meshheading:2186029-Cloning, Molecular, pubmed-meshheading:2186029-Cricetinae, pubmed-meshheading:2186029-DNA, pubmed-meshheading:2186029-Drosophila, pubmed-meshheading:2186029-Escherichia coli, pubmed-meshheading:2186029-Fluorescent Antibody Technique, pubmed-meshheading:2186029-Gene Expression, pubmed-meshheading:2186029-Membrane Proteins, pubmed-meshheading:2186029-Mesocricetus, pubmed-meshheading:2186029-Molecular Sequence Data, pubmed-meshheading:2186029-Molecular Weight, pubmed-meshheading:2186029-Nuclear Envelope, pubmed-meshheading:2186029-Nuclear Proteins, pubmed-meshheading:2186029-Nucleic Acid Hybridization, pubmed-meshheading:2186029-Plasmids, pubmed-meshheading:2186029-Protein Biosynthesis, pubmed-meshheading:2186029-Restriction Mapping, pubmed-meshheading:2186029-Transfection
pubmed:year
1990
pubmed:articleTitle
Isolation and characterization of the Drosophila nuclear envelope otefin cDNA.
pubmed:affiliation
Department of Genetics, Hebrew University of Jerusalem, Israel.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't